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Home >> Antibodies >> FGG Antibody / Fibrinogen gamma chain / FIBG

FGG Antibody / Fibrinogen gamma chain / FIBG (F54860)

  Catalog No Formulation Size Price (USD)  
Image F54860-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54860-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human skeletal muscle tissue with FGG antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human MDA-MB-231 cells with FGG antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human A431 cell lysate with FGG antibody. Predicted molecular weight ~52 kDa.
Western blot testing of human NIC-H460 cell lysate with FGG antibody. Predicted molecular weight ~52 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P02679
Applications Immunohistochemistry (FFPE) : 1:10-1:50
Immunofluorescence : 1:10-1:50
Western blot : 1:500-1:1000
Limitations This FGG antibody is available for research use only.
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Description

FIBG is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in its gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia.

Application Notes

The stated application concentrations are suggested starting points. Titration of the FGG antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 420-449 from the human protein was used as the immunogen for the FGG antibody.

Storage

Aliquot the FGG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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