FGFBP1 Antibody / Fibroblast growth factor-binding protein 1 Antibody (FY13532)

FGFBP1 Antibody by IHC in human liver carcinoma. Immunohistochemistry of paraffin-embedded human liver carcinoma using Fibroblast growth factor-binding protein 1 Antibody reveals brown staining in tumor cells, with blue hematoxylin counterstaining providing nuclear contrast. Heat-induced antigen retrieval was performed in EDTA buffer at pH 8.0. Tissue sections were blocked with 10% goat serum and incubated with primary antibody at 2 ug/mL overnight at 4oC. Peroxidase-linked secondary detection with DAB chromogen was used to visualize FGFBP1-associated immunoreactivity in the tumor section.

FGFBP1 Antibody by WB in human A431 and PC-3 cells. Western blot analysis with Fibroblast growth factor-binding protein 1 Antibody identifies immunoreactive bands near 26 kDa in A431 cells (lane 1) and PC-3 cells (lane 2), consistent with the calculated molecular weight of approximately 26.3 kDa. Reduced whole-cell lysates containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. Membranes were blocked in 5% non-fat milk and incubated with primary antibody at 0.5 ug/mL overnight at 4oC. An HRP-conjugated secondary antibody and chemiluminescent substrate were used for detection.

FGFBP1 Antibody by FACS in human PC-3 cells. Flow cytometric analysis with Fibroblast growth factor-binding protein 1 Antibody shows a rightward fluorescence shift in stained PC-3 cells (blue trace) relative to the rabbit IgG isotype control (green) and unstained control (red). Cells were fixed with 4% paraformaldehyde, blocked with 10% goat serum, and incubated with primary antibody at 1 ug per 1 x 10^6 cells for 30 minutes at 20oC. DyLight 488-conjugated secondary antibody was applied before fluorescence acquisition. The separation of the traces demonstrates antibody-associated signal under these staining conditions.