FGF-23 Antibody - Protein Microarray Validated FGF23/4169 / Fibroblast Growth Factor 23 [clone FGF23/4169] (V9587)

FGF-23 Antibody Protein Microarray Validated FGF23/4169 immunohistochemistry staining of human kidney tissue. IHC analysis of FFPE human kidney was performed using microarray validated FGF-23 antibody clone FGF23/4169 at 2 ug/ml in PBS for 30 min at RT. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before staining. HRP-DAB brown staining highlights epithelial cells within renal tubules, consistent with detection of Fibroblast growth factor 23 / FGF23. Inset shows PBS used in place of primary antibody as a secondary antibody negative control.

SDS-PAGE analysis of purified, BSA-free FGF-23 antibody (clone FGF23/4169) as confirmation of integrity and purity.

FGF-23 Antibody Protein Microarray Validated FGF23/4169 specificity analysis on HuProt microarray. Protein microarray screening was performed using FGF-23 Antibody Protein Microarray Validated clone FGF23/4169 against the HuProt(TM) human protein microarray containing more than 19,000 full-length human proteins. The antibody shows a strong and specific signal for Fibroblast growth factor 23 / FGF23 with the highest Z-score and S-score values among all proteins on the array, demonstrating high specificity of clone FGF23/4169 for its intended target. Z-score represents the signal intensity produced when the antibody binds a particular protein on the array relative to the overall signal distribution, expressed in standard deviations above the mean. The S-score reflects the difference between adjacent Z-scores when proteins are ranked by signal strength and provides a measure of relative antibody specificity for the target protein.