FFAR3 Antibody / Free fatty acid receptor 3 Antibody (FY13519)

FFAR3 Antibody by FACS in SH-SY5Y cells. Flow cytometry analysis of fixed SH-SY5Y cells using Free fatty acid receptor 3 Antibody shows a clear rightward shift of the antibody-stained population (blue) relative to the rabbit IgG isotype control (green) and unstained blank (red). Cells were fixed with 4% paraformaldehyde, blocked with normal goat serum, and incubated with 1 ug primary antibody per 1 x 10^6 cells. Fluorescent anti-rabbit secondary antibody was used for detection. The histogram demonstrates FFAR3-associated staining under these fixed-cell assay conditions.

FFAR3 Antibody by WB in four human cell lines. Western blot analysis using Free fatty acid receptor 3 Antibody shows immunoreactivity near 38-40 kDa in K562 (lane 1), SH-SY5Y (lane 2), U251 (lane 3), and HepG2 (lane 4) whole-cell lysates. Thirty ug of reduced protein was loaded per lane on a 12% SDS-PAGE gel. The primary antibody was applied at 0.5 ug/mL overnight at 4oC, followed by HRP-linked secondary antibody detection. Additional bands are visible at higher and lower apparent molecular weights, but their identities are not established by the presented blot. The signal near 38-40 kDa corresponds to the band labeled FFAR3.