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Home >> Antibodies >> FEZF1 Antibody

FEZF1 Antibody (RQ6176)

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  Catalog No Formulation Size Price (USD)  
Image RQ6176 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human PC-3 cells with FEZF1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human PC-3 cells with FEZF1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human SH-SY5Y, 2) rat brain and 3) mouse brain lysate with FEZF1 antibody. Predicted molecular weight ~ 52 kDa.
Flow cytometry testing of human U-2 OS cells with FEZF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FEZF1 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.0125% sodium azide
UniProt A0PJY2
Applications Western blot : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Immunofluorescence : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This FEZF1 antibody is available for research use only.
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  • Applications : WB, ELISA
    Reactivity : Human, Mouse

Description

FEZ family zinc finger 1 is a protein that in humans is encoded by the FEZF1 gene. This gene encodes a transcriptional repressor that belongs to the zinc finger double domain protein family. The encoded protein is thought to play a role in the embryonic migration of gonadotropin-releasing hormone neurons into the brain. Mutations in this gene are associated with hypogonadotropic hypogonadism-22 with anosmia. Alternative splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the FEZF1 antibody should be determined by the researcher.

Immunogen

A human recombinant partial protein (amino acids A45-K258) was used as the immunogen for the FEZF1 antibody.

Storage

After reconstitution, the FEZF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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