Ferroportin 1 Antibody | Ferroportin Antibody

	Catalog No	Formulation	Size	Price (USD)
	RQ7663	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

Ferroportin 1 Antibody Human Ovarian Cancer IF. Immunofluorescent staining of FFPE human ovarian cancer tissue demonstrates ferroportin expression in green with DAPI nuclear counterstaining in blue. Heat-induced epitope retrieval was performed by steaming sections in pH8 EDTA buffer for 20 minutes. Fluorescence is observed predominantly in the cytoplasmic and cell membrane regions of tumor cells, consistent with the localization of ferroportin as a transmembrane iron exporter. This Ferroportin 1 Antibody, also called Ferroportin Antibody, supports studies of cellular iron export, iron homeostasis, and cancer metabolism.

Ferroportin 1 Antibody Human Prostate Cancer IHC. Immunohistochemical staining of FFPE human prostate adenocarcinoma tissue demonstrates ferroportin expression following heat-induced epitope retrieval in pH8 EDTA buffer for 20 minutes. Brown DAB staining is observed in tumor cells, with predominantly cytoplasmic and membranous localization. Nuclei are counterstained blue with hematoxylin. This Ferroportin 1 Antibody, also called Ferroportin Antibody, supports studies of cellular iron export, iron homeostasis, and prostate cancer metabolism.

Ferroportin 1 Antibody Human Rectal Cancer IHC. Immunohistochemical staining of FFPE human rectum adenocarcinoma tissue demonstrates ferroportin expression following heat-induced epitope retrieval in pH8 EDTA buffer for 20 minutes. Brown DAB staining is observed predominantly in the cytoplasmic and membranous regions of malignant glandular epithelial cells. Nuclei are counterstained blue with hematoxylin. This Ferroportin 1 Antibody, also called Ferroportin Antibody, supports studies of cellular iron export, iron homeostasis, and colorectal cancer metabolism.

Ferroportin 1 Antibody Human Ovarian Cancer IHC. Immunohistochemical staining of FFPE human ovarian serous adenocarcinoma tissue demonstrates ferroportin expression following heat-induced epitope retrieval in pH8 EDTA buffer for 20 minutes. Brown DAB staining is observed predominantly in the cytoplasmic and membranous regions of tumor cells. Nuclei are counterstained blue with hematoxylin. This Ferroportin 1 Antibody, also called Ferroportin Antibody, supports studies of cellular iron export, iron homeostasis, and ovarian cancer metabolism.

Ferroportin 1 Antibody Human Laryngeal Cancer IHC. Immunohistochemical staining of FFPE human larynx squamous cell carcinoma tissue demonstrates ferroportin expression following heat-induced epitope retrieval in pH8 EDTA buffer for 20 minutes. Brown DAB staining is observed predominantly in the cytoplasmic and membranous regions of tumor cells. Nuclei are counterstained blue with hematoxylin. This Ferroportin 1 Antibody, also called Ferroportin Antibody, supports studies of cellular iron export, iron homeostasis, and laryngeal cancer metabolism.

Ferroportin 1 Antibody Human Liver Cancer IHC. Immunohistochemical staining of FFPE human liver cancer tissue demonstrates ferroportin expression following heat-induced epitope retrieval in pH8 EDTA buffer for 20 minutes. Brown DAB staining is observed predominantly in the cytoplasmic and membranous regions of tumor cells. Nuclei are counterstained blue with hematoxylin. This Ferroportin 1 Antibody, also called Ferroportin Antibody, supports studies of cellular iron export, iron homeostasis, and liver cancer metabolism.

Ferroportin 1 Antibody Human, Rat and Mouse WB. Western blot testing of human plasma (lane 1), rat liver (lane 2), mouse spleen (lane 3), and mouse liver (lane 4) demonstrates ferroportin detection at approximately 65â70 kDa. The predicted molecular weight of ferroportin is approximately 62 kDa, with higher apparent molecular weights possible due to glycosylation. This Ferroportin 1 Antibody, also called Ferroportin Antibody, supports studies of iron transport, cellular iron export, and systemic iron homeostasis.

Ferroportin 1 Antibody U-2 OS Cell FACS. Flow cytometry analysis of human U-2 OS cells with Ferroportin 1 antibody at 1 ug/million cells demonstrates a clear fluorescence shift in the antibody-stained population (blue) relative to the isotype control (green). Cells alone are shown in red, and samples were blocked with goat serum before staining. This Ferroportin 1 Antibody, also called Ferroportin Antibody, supports studies of cellular iron export, iron homeostasis, and ferroportin expression.

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Ferroportin 1 Antibody Human Ovarian Cancer IF. Immunofluorescent staining of FFPE human ovarian cancer tissue demonstrates ferroportin expression in green with DAPI nuclear counterstaining in blue. Heat-induced epitope retrieval was performed by steaming sections in pH8 EDTA buffer for 20 minutes. Fluorescence is observed predominantly in the cytoplasmic and cell membrane regions of tumor cells, consistent with the localization of ferroportin as a transmembrane iron exporter. This Ferroportin 1 Antibody, also called Ferroportin Antibody, supports studies of cellular iron export, iron homeostasis, and cancer metabolism.

Ferroportin 1 Antibody Human Prostate Cancer IHC. Immunohistochemical staining of FFPE human prostate adenocarcinoma tissue demonstrates ferroportin expression following heat-induced epitope retrieval in pH8 EDTA buffer for 20 minutes. Brown DAB staining is observed in tumor cells, with predominantly cytoplasmic and membranous localization. Nuclei are counterstained blue with hematoxylin. This Ferroportin 1 Antibody, also called Ferroportin Antibody, supports studies of cellular iron export, iron homeostasis, and prostate cancer metabolism.

Ferroportin 1 Antibody Human Rectal Cancer IHC. Immunohistochemical staining of FFPE human rectum adenocarcinoma tissue demonstrates ferroportin expression following heat-induced epitope retrieval in pH8 EDTA buffer for 20 minutes. Brown DAB staining is observed predominantly in the cytoplasmic and membranous regions of malignant glandular epithelial cells. Nuclei are counterstained blue with hematoxylin. This Ferroportin 1 Antibody, also called Ferroportin Antibody, supports studies of cellular iron export, iron homeostasis, and colorectal cancer metabolism.

Ferroportin 1 Antibody Human Ovarian Cancer IHC. Immunohistochemical staining of FFPE human ovarian serous adenocarcinoma tissue demonstrates ferroportin expression following heat-induced epitope retrieval in pH8 EDTA buffer for 20 minutes. Brown DAB staining is observed predominantly in the cytoplasmic and membranous regions of tumor cells. Nuclei are counterstained blue with hematoxylin. This Ferroportin 1 Antibody, also called Ferroportin Antibody, supports studies of cellular iron export, iron homeostasis, and ovarian cancer metabolism.

Ferroportin 1 Antibody Human Laryngeal Cancer IHC. Immunohistochemical staining of FFPE human larynx squamous cell carcinoma tissue demonstrates ferroportin expression following heat-induced epitope retrieval in pH8 EDTA buffer for 20 minutes. Brown DAB staining is observed predominantly in the cytoplasmic and membranous regions of tumor cells. Nuclei are counterstained blue with hematoxylin. This Ferroportin 1 Antibody, also called Ferroportin Antibody, supports studies of cellular iron export, iron homeostasis, and laryngeal cancer metabolism.

Ferroportin 1 Antibody Human Liver Cancer IHC. Immunohistochemical staining of FFPE human liver cancer tissue demonstrates ferroportin expression following heat-induced epitope retrieval in pH8 EDTA buffer for 20 minutes. Brown DAB staining is observed predominantly in the cytoplasmic and membranous regions of tumor cells. Nuclei are counterstained blue with hematoxylin. This Ferroportin 1 Antibody, also called Ferroportin Antibody, supports studies of cellular iron export, iron homeostasis, and liver cancer metabolism.

Ferroportin 1 Antibody Human, Rat and Mouse WB. Western blot testing of human plasma (lane 1), rat liver (lane 2), mouse spleen (lane 3), and mouse liver (lane 4) demonstrates ferroportin detection at approximately 65–70 kDa. The predicted molecular weight of ferroportin is approximately 62 kDa, with higher apparent molecular weights possible due to glycosylation. This Ferroportin 1 Antibody, also called Ferroportin Antibody, supports studies of iron transport, cellular iron export, and systemic iron homeostasis.

Ferroportin 1 Antibody U-2 OS Cell FACS. Flow cytometry analysis of human U-2 OS cells with Ferroportin 1 antibody at 1 ug/million cells demonstrates a clear fluorescence shift in the antibody-stained population (blue) relative to the isotype control (green). Cells alone are shown in red, and samples were blocked with goat serum before staining. This Ferroportin 1 Antibody, also called Ferroportin Antibody, supports studies of cellular iron export, iron homeostasis, and ferroportin expression.

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	Q9NP59
RRID	AB_3753598
Localization	Cell membrane, cytoplasm
Applications	Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Flow Cytometry : 1-3ug/million cells Immunofluorescence : 5ug/ml Direct ELISA : 0.1-0.5ug/ml
Limitations	This Ferroportin 1 Antibody / Ferroportin Antibody is available for research use only.
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Description

Ferroportin 1 Antibody recognizes ferroportin, an iron-exporting transmembrane protein encoded by the SLC40A1 gene. Also known as FPN, FPN1, IREG1, and MTP1, ferroportin plays a central role in maintaining cellular and systemic iron homeostasis. It is highly expressed in cells responsible for transferring iron into the circulation, including intestinal enterocytes, macrophages that recycle iron from senescent erythrocytes, hepatocytes that store iron, and placental cells involved in maternal-fetal iron transport. By exporting ferrous iron across the plasma membrane, ferroportin provides a critical connection between intracellular iron metabolism and circulating iron availability.

Ferroportin activity is tightly regulated by hepcidin, a peptide hormone produced primarily by the liver. Hepcidin binds ferroportin at the cell surface and regulates its iron-export activity, thereby reducing the movement of iron into plasma. This hepcidin-ferroportin regulatory axis is fundamental to systemic iron balance and responds to factors including body iron stores, erythropoietic demand, hypoxia, and inflammation. A Ferroportin Antibody can therefore support investigations of iron transport, hepcidin signaling, macrophage iron recycling, intestinal iron absorption, and the mechanisms controlling circulating iron availability.

Alterations in ferroportin expression or function are associated with disorders of iron metabolism. Pathogenic variants in SLC40A1 can cause ferroportin disease, an inherited form of iron overload characterized by abnormal iron accumulation and altered iron distribution among tissues. Dysregulation of the hepcidin-ferroportin pathway also contributes to anemia of inflammation and other conditions involving disturbed iron availability. Ferroportin has additionally become an important target in cancer and immunometabolism research because iron availability can influence oxidative metabolism, cellular proliferation, immune cell function, and susceptibility to iron-dependent forms of cell death. A Ferroportin 1 Antibody can be useful for examining changes in ferroportin expression across these physiological and disease models.

Research on ferroportin also intersects with studies of ferritin, transferrin, transferrin receptor, heme metabolism, macrophage biology, and ferroptosis. Because ferroportin represents the major pathway for cellular iron export, changes in its abundance or localization can substantially alter intracellular iron pools and downstream iron-dependent processes. NSJ Bioreagents offers antibodies for investigating proteins involved in iron metabolism and related cellular pathways. A Ferroportin 1 Antibody is a useful research reagent for studying ferroportin expression, SLC40A1 biology, cellular iron export, hepcidin regulation, systemic iron homeostasis, and iron-associated disease mechanisms.

Explore our Metabolism Antibodies page to find additional antibodies for studying iron homeostasis, nutrient metabolism, and metabolic regulation.

Application Notes

Optimal dilution of the Ferroportin 1 Antibody / Ferroportin Antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-V571) was used as the immunogen for the Ferroportin 1 antibody.

Storage

After reconstitution, the Ferroportin 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

Ferroportin 1 antibody, Ferroportin antibody, SLC40A1 antibody, FPN antibody, FPN1 antibody, Solute Carrier Family 40 Member 1 antibody, Iron-Regulated Transporter 1 antibody

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Ferroportin 1 Antibody / Ferroportin Antibody (RQ7663)

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