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Home >> Antibodies >> Ferritin heavy chain Antibody / FTH1

Ferritin heavy chain Antibody / FTH1 (F54896)

  Catalog No Formulation Size Price (USD)  
Image F54896-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54896-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human 1) HeLa and 2) HT-29 cell lysate with Ferritin heavy chain antibody. Predicted molecular weight ~21 kDa.
Western blot testing of human A2058 cell lysate with Ferritin heavy chain antibody. Predicted molecular weight ~21 kDa.
IHC testing of FFPE human hepatocellular carcinoma tissue with Ferritin heavy chain antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HepG2 cells with Ferritin heavy chain antibody; Blue=isotype control, Green= Ferritin heavy chain antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P02794
Localization Cytoplasmic
Applications Flow cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:50-1:100
Western blot : 1:500-1:1000
Limitations This Ferritin heavy chain antibody is available for research use only.
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Description

FTH1 is the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Ferritin heavy chain antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 154-183 from the human protein was used as the immunogen for the Ferritin heavy chain antibody.

Storage

Aliquot the Ferritin heavy chain antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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