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Home >> Antibodies >> Ferritin Antibody (light chain specific)

Ferritin Antibody (light chain specific) [clone FTLC1-1] (V7209)

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  Catalog No Formulation Size Price (USD)  
Image V7209-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7209-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7209SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Western blot testing of human HeLa cell lysate with Ferritin antibody (clone FTLC1-1). Predicted molecular weight: ~20 kDa.
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Western blot testing of human HeLa cell lysate with Ferritin antibody (clone FTLC1-1). Predicted molecular weight: ~20 kDa.
IHC staining of FFPE human pancreas with recombinant Ferritin antibody (clone FTLC1-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name FTLC1-1
Purity Protein G affinity chromatography
Gene ID 2512
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Ferritin antibody is available for research use only.
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Description

Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ferritin antibody to be titered up or down for optimal performance.

Immunogen

Amino acids 38-165 of human FTL were used as the immunogen for this Ferritin antibody (light chain specific).

Storage

Store the Ferritin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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