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Home >> Antibodies >> FCER2 Antibody for IHC / Fc epsilon receptor 2

FCER2 Antibody for IHC / Fc epsilon receptor 2 [clone MSVA-023M] (V6074)

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  Catalog No Formulation Size Price (USD)  
Image V6074-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 654.50
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V6074-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
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Immunohistochemistry of FCER2 Antibody for IHC (clone MSVA-023M) in human tissue microarrays. FFPE human normal and cancer tissues were stained with FCER2 antibody for IHC (clone MSVA-023M). In the normal tissue gallery, strong membranous HRP-DAB brown staining is observed in B lymphocytes within tonsil and lymph node germinal centers, consistent with known Fc epsilon receptor 2 expression on mature follicular B cells. Most non-lymphoid tissues show minimal to absent staining, with only scattered lymphoid cells demonstrating positivity. In the cancer tissue gallery, strong membranous staining is observed in CD23 positive B cell lymphomas, while most non-lymphoid malignancies show little to no expression. The staining distribution is consistent with the expected tissue specific expression profile of FCER2 as reported in Human Protein Atlas.
Species Reactivity Human
Format Purified
Host Mouse
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name MSVA-023M
UniProt P06734
Localization Cell membrane, Secreted
Applications Immunohistochemistry (FFPE) : 1:100-1:200
Limitations This FCER2/Fc epsilon receptor 2 antibody is available for research use only.
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Description

FCER2 Antibody for IHC recognizes Fc epsilon receptor 2, a type II transmembrane glycoprotein encoded by the FCER2 gene on chromosome 19p13.3. Fc epsilon receptor 2 is also widely known as CD23 or Low affinity immunoglobulin epsilon Fc receptor and is a member of the C-type lectin family. It functions as the low affinity receptor for IgE and plays an important role in regulating IgE mediated immune responses, B cell activation, and humoral immunity. FCER2 is primarily expressed on mature B lymphocytes and certain activated immune cell subsets within secondary lymphoid tissues.

Structurally, Fc epsilon receptor 2 contains a short N-terminal cytoplasmic domain, a single transmembrane segment, and a large extracellular C-type lectin-like domain responsible for IgE binding. In addition to its membrane bound form, FCER2 can be proteolytically cleaved to generate soluble fragments that retain biologic activity and influence immune signaling pathways. Through interactions with IgE and CD21, FCER2 participates in antigen presentation, modulation of IgE synthesis, and regulation of B cell proliferation and differentiation. Subcellular localization is predominantly membranous, often accompanied by variable cytoplasmic staining depending on cellular activation state.

In normal tissues, FCER2 expression is most prominent in tonsil, lymph node, and spleen, where it is characteristically expressed by follicular B cells in germinal centers and mantle zones. Immunohistochemical staining typically highlights follicular architecture with strong membranous labeling in B cell rich regions and minimal staining in T cell predominant interfollicular areas. Because of this distinct expression pattern, FCER2 antibody is widely used in research settings focused on B cell biology and germinal center dynamics.

Altered expression of Fc epsilon receptor 2 has been associated with allergic diseases, asthma, and certain B cell lymphoproliferative disorders such as chronic lymphocytic leukemia. A mouse monoclonal antibody such as clone MSVA-023M provides a consistent tool for detecting FCER2 in formalin-fixed, paraffin-embedded tissues. FCER2 Antibody for IHC is suitable for detecting Fc epsilon receptor 2 expression in research applications involving immunology and lymphoid tissue studies.

Application Notes

1. Optimal dilution of the FCER2/Fc epsilon receptor 2 antibody for IHC should be determined by the researcher.

2. This FCER2/Fc epsilon receptor 2 antibody is recombinantly produced by expression in human HEK293 cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Immunogen

A recombinant fragment (around amino acids 48-321) of human FCER2/CD23 protein (exact sequence is proprietary) was used as the immunogen for the FCER2/Fc epsilon receptor 2 antibody for IHC.

Storage

FCER2/Fc epsilon receptor 2 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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