FBXW8 Antibody / F-box/WD repeat-containing protein 8 (FY12997)

Western blot analysis of FBXW8 using anti-FBXW8 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human whole cell lysates, Lane 4: rat small intestine tissue lysates, Lane 5: rat lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBXW8 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. A specific band was detected for FBXW8 at approximately 67 kDa. The expected molecular weight of FBXW8 is ~67 kDa.