FATE1 Antibody / Fetal and adult testis expressed 1 Antibody (FY13655)

FATE1 Antibody by WB in 4 lysate samples. Western blot analysis using FATE1 Antibody was performed with 30 ug of protein per lane under reducing conditions on a 12% SDS-PAGE gel. Lane assignments: 1, human HeLa whole-cell lysate; 2, rat testis lysate; 3, rat C6 whole-cell lysate; 4, mouse RAW264.7 whole-cell lysate. Following electrophoresis, proteins were transferred to nitrocellulose and blocked with 5% non-fat milk. The membrane was incubated with rabbit primary antibody at 0.5 ug/ml overnight at 4oC and HRP-conjugated goat anti-rabbit IgG secondary antibody (1:5000). ECL detection showed an immunoreactive band near 24 kDa, as shown in the figure. The higher apparent mass should not be attributed to a specific modification without independent supporting evidence.

FATE1 Antibody by IHC in Human Liver Cancer Tissue. Immunohistochemistry analysis of FATE1 expression in a paraffin-embedded section of human liver cancer tissue using FATE1 Antibody. Brown DAB staining is observed in numerous tumor cells, predominantly within the cytoplasm, with heterogeneous staining intensity across the tissue. Nuclei are counterstained with hematoxylin, and surrounding stromal regions show comparatively limited immunoreactivity. The staining pattern is consistent with cytoplasmic detection of FATE1 in the examined tumor section.