FASLG Antibody / CD178 Antibody (FY12296)

FASLG Antibody Detection in Human Jurkat T Lymphocytes FACS. Flow cytometry analysis of human Jurkat T lymphocytes using FASLG Antibody shows a clear rightward fluorescence shift in the antibody-stained population (blue) relative to the rabbit IgG isotype control (green) and unlabeled cells (red). Cells were fixed with 4% paraformaldehyde, blocked with 10% normal goat serum, and incubated with primary antibody at 1 ug/million cells for 30 min at 20oC. The distinct population separation demonstrates detection of Fas ligand, also known as CD178, in Jurkat cells.

FASLG Antibody Detects Fas Ligand in Human Jurkat T Lymphocytes WB. Western blot analysis of human Jurkat T-lymphocyte whole-cell lysate using FASLG Antibody shows a prominent band at approximately 31 kDa, consistent with the expected molecular weight of FASLG. Proteins were separated by 10% SDS-PAGE, transferred to nitrocellulose, and the membrane was incubated with primary antibody at 0.5 ug/ml overnight at 4oC before HRP-based detection. The major band demonstrates detection of Fas ligand/CD178 in Jurkat cell lysate, with comparatively limited additional background signal.