FasL Antibody FASLG/4453 / TNFSF6 Antibody [clone FASLG/4453] (V9541)

FasL Antibody Staining of Human Tonsil Lymphocytes IHC. Immunohistochemistry staining of FFPE human tonsil tissue with FasL Antibody (clone FASLG/4453) shows distinct brown staining in subsets of lymphocytes, with positive cells distributed primarily through the peripheral portion of the lymphoid region shown. HIER was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min followed by cooling before testing. The heterogeneous cellular staining demonstrates detection of Fas ligand, also known as TNFSF6, in human tonsil tissue.

FasL Antibody Purity and Integrity by SDS-PAGE. SDS-PAGE analysis of 2 ug purified, BSA-free FasL Antibody (clone FASLG/4453) shows the intact antibody migrating at approximately 150 kDa under non-reducing conditions. Under reducing conditions, distinct heavy and light immunoglobulin chains are visible at approximately 50 kDa and 25 kDa, respectively. The expected migration pattern supports the integrity and purity of this TNFSF6 Antibody preparation.

FasL Antibody Protein Microarray Validated. HuProt protein microarray analysis of FasL Antibody (clone FASLG/4453) against more than 19,000 full-length human proteins identifies FASLG as the dominant target, with a Z-score of 110.8 and S-score of 87.1. The Z-score represents signal strength in standard deviations above the array mean, while the S-score measures separation from the next-ranked protein. FASLG is clearly separated from the secondary signals, providing strong evidence of preferential recognition of FASLG/TNFSF6 under the tested microarray conditions.