Fas Ligand Antibody FASLG/4455 / CD95L Antibody [clone FASLG/4455] (V8610)

Fas Ligand Antibody Staining of Human Tonsil Lymphocytes IHC. Immunohistochemistry staining of FFPE human tonsil tissue with Fas Ligand Antibody (clone FASLG/4455) shows distinct brown staining in subsets of lymphocytes, with prominent signal concentrated within the lymphoid compartment. Staining is predominantly cytoplasmic and membranous, while surrounding areas show comparatively little signal. HIER was performed by boiling sections in pH 9 10mM Tris with 1mM EDTA for 20 min followed by cooling at room temperature. The staining pattern demonstrates detection of Fas ligand, also known as CD95L, in human tonsil tissue.

Fas Ligand Antibody Detection in Human Tonsil Tissue IHC. Immunohistochemistry staining of FFPE human tonsil tissue with Fas Ligand Antibody (clone FASLG/4455) shows heterogeneous brown staining among lymphocytes, with numerous positive cells displaying cytoplasmic and membranous signal against a largely negative background. HIER was performed in pH 9 Tris-EDTA buffer for 20 min followed by cooling before staining. This second tonsil field provides complementary evidence of CD95L expression within human lymphoid tissue.

Fas Ligand Antibody Protein Microarray Validated. HuProt protein microarray analysis of Fas Ligand Antibody (clone FASLG/4455) against more than 19,000 full-length human proteins identifies FASLG as the top-ranked target with a Z-score of 116.67 and an S-score of 110.28. The Z-score reflects signal strength in standard deviations above the array mean, while the S-score measures separation between FASLG and the next-ranked protein. The exceptionally large separation visible in the plot provides strong evidence of preferential recognition of FASLG/CD95L under the tested microarray conditions.