Fas Ligand Antibody / Microarray Specificity Validated Antibody [clone FASLG/4455] (V8610)

Fas Ligand Antibody Tonsil IHC. Immunohistochemistry analysis of Fas ligand / FASLG expression in FFPE human tonsil tissue using mouse monoclonal antibody clone FASLG/4455. HRP-DAB brown staining is observed in scattered lymphocytes within interfollicular regions and germinal center-associated areas, showing predominantly cytoplasmic and membranous localization, while surrounding stromal elements remain largely negative; nuclei are counterstained blue. Antigen retrieval was performed by boiling sections in 10 mM Tris buffer with 1 mM EDTA, pH 9, for 20 min followed by cooling at room temperature.

Fas Ligand Antibody Human Tonsil Tissue IHC. Immunohistochemical detection of Fas ligand / CD95L expression in formalin-fixed, paraffin-embedded human tonsil using FASLG antibody, clone FASLG/4455. Distinct HRP-DAB brown signal highlights subsets of activated lymphocytes with cytoplasmic and membranous staining patterns, particularly within lymphoid compartments, while background staining remains minimal; nuclei are counterstained blue. Heat-induced epitope retrieval was carried out in pH 9 Tris-EDTA buffer for 20 min with subsequent cooling prior to staining.

Fas Ligand Antibody Protein Microarray Validation. HuProt microarray analysis of Fas ligand / FASLG specificity using FASLG antibody, clone FASLG/4455, against more than 19,000 full-length human proteins. FASLG is identified as the top-ranked target by Z score with a strong signal intensity and clear separation from the next highest proteins, resulting in a high S score that supports selective target recognition. These results demonstrate preferential binding to Fas ligand with minimal off-target interaction under the tested conditions.