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Home >> Antibodies >> FAS-associated factor 1 Antibody / FAF1

FAS-associated factor 1 Antibody / FAF1 (RQ7977)

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  Catalog No Formulation Size Price (USD)  
Image RQ7977 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A549 cells with FAS-associated factor 1 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A549 cells with FAS-associated factor 1 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human 293T, 3) human K562, 4) human ThP-1, 5) rat brain, 6) rat testis, 7) mouse brain and 8) mouse testis tissue lysate with FAS-associated factor 1 antibody. Expected molecular weight ~74 kDa.
Flow cytometry testing of human K562 cells with FAS-associated factor 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= FAS-associated factor 1 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9UNN5
Localization Nuclear
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This FAS-associated factor 1 antibody is available for research use only.
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Description

Fas-associated factor-1 (FAF1) is a Fas-binding pro-apoptotic protein that is a component of the death-inducing signaling complex in Fas-mediated apoptosis. FAF1 is involved in negative regulation of NF-kappaB activation. In addition to the previously known function as a component of the Fas death-inducing signaling complex, i.e. NF-kappaB activity suppressor by cytoplasmic retention of NF-kappaB p65 via physical interaction. hFAF1 was expressed abundantly in testis, skeletal muscle, and heart as 2.8 kb mRNA. Polyclonal antibody against hFAF1 detected 74 kD protein, a deduced protein size from the ORF and 40 kD protein in some cell lines.

Application Notes

Optimal dilution of the FAS-associated factor 1 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids R91-E650) was used as the immunogen for the FAS-associated factor 1 antibody.

Storage

After reconstitution, the FAS-associated factor 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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