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Home >> Antibodies >> FAK Antibody / Focal Adhesion Kinase / PTK2

FAK Antibody / Focal Adhesion Kinase / PTK2 (R32191)

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Image R32191 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat lung, 2) mouse lung, 3) rat brain, 4) human U20S, 5) human HeLa, 6) mouse NIH3T3 lysate with FAK antibody. FAK has numerous isoforms and is routinely observed in western blot between 80~125 kDa.
Western blot testing of 1) rat lung, 2) mouse lung, 3) rat brain, 4) human U20S, 5) human HeLa, 6) mouse NIH3T3 lysate with FAK antibody. FAK has numerous isoforms and is routinely observed in western blot between 80~125 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt Q05397
Applications Western blot : 0.1-0.5ug/ml
Limitations This FAK antibody is available for research use only.
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Description

PTK2 protein tyrosine kinase 2 (PTK2), also known as Focal Adhesion Kinase (FAK), is a protein that, in humans, is encoded by the PTK2 gene. This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined.

Application Notes

Optimal dilution of the FAK antibody should be determined by the researcher.

Immunogen

Amino acids AHALAVDAKNLLDVIDQARLKMLGQTRPH of human PTK2/FAK were used as the immunogen for the FAK antibody.

Storage

After reconstitution, the FAK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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