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Home >> Antibodies >> FADD Antibody

FADD Antibody [clone 5E8-A7] (F54009)

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  Catalog No Formulation Size Price (USD)  
Image F54009-0.1ML In PBS with 50% glycerol and 0.03% ProClin 300 0.1 ml 399
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Western blot testing of SW480, Jurkat and HeLa cell lysates using FADD antibody at 1:500. Predicted molecular weight ~28 kDa.
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Western blot testing of SW480, Jurkat and HeLa cell lysates using FADD antibody at 1:500. Predicted molecular weight ~28 kDa.
Immunoprecipitation of FADD from HeLa cell lysate using FADD antibody. The precipitate was subsequently immunoblotted with the same mAb.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 5E8-A7
Purity Protein G affinity
UniProt Q13158
Applications Western blot : 1:500
Immunoprecipitation : suitable
Limitations This FADD antibody is available for research use only.
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Description

Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the FADD antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A human recombinant protein was used as the immunogen for this FADD antibody.

Storage

Store the FADD antibody at -20oC.

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