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Home >> Antibodies >> FADD Antibody

FADD Antibody [clone 5A3-C7-G9] (F54022)

  Catalog No Formulation Size Price (USD)  
Image F54022-0.1ML In PBS with 50% glycerol and 0.03% ProClin 300 0.1 ml 399
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Immunoprecipitation of FADD from HeLa cell lysate using FADD antibody. The precipitate was subsequently immunoblotted with the same mAb.
Western blot testing of SW480, Jurkat and HeLa cell lysates using FADD antibody at 1:500. Predicted molecular weight ~28 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a
Clone Name 5A3-C7-G9
Purity Protein G affinity
UniProt Q13158
Applications Western blot : 1:500
Immunoprecipitation : suitable
Limitations This FADD antibody is available for research use only.
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Description

Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the FADD antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A human recombinant protein was used as the immunogen for this FADD antibody.

Storage

Store the FADD antibody at -20oC.

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