Factor XIIIa Antibody - Protein Microarray Validated [clone F13A1/1683] (V3500)

Description

Factor XIIIa Antibody Protein Microarray Validated clone F13A1/1683 recognizes Factor XIII A chain, the catalytic transglutaminase subunit encoded by the F13A1 gene on chromosome 6p25.1. Factor XIII A chain is a cytoplasmic enzyme expressed in platelets, macrophages, dermal dendritic cells, and selected stromal populations. Upon thrombin and calcium activation, the zymogen form of coagulation factor XIII is cleaved to generate active Factor XIIIa, which stabilizes fibrin clots by catalyzing covalent cross-linking between fibrin monomers. This cross-linking step is essential for clot integrity and resistance to fibrinolysis.

Factor XIII A chain belongs to the transglutaminase family and contains a catalytic core domain that forms epsilon-gamma glutamyl-lysine isopeptide bonds between substrate proteins. In plasma, coagulation factor XIII circulates as a heterotetramer composed of two catalytic A subunits and two carrier B subunits. Following proteolytic activation, the B subunits dissociate and the A subunits become enzymatically active. Beyond coagulation, Factor XIIIa participates in extracellular matrix remodeling by cross-linking fibronectin, collagen, and other structural proteins, supporting tissue repair and wound healing.

In normal tissues, Factor XIII A chain expression is prominent in dermal dendrocytes and tissue macrophages. It is also detectable in placenta and bone marrow-derived cells. In research and diagnostic pathology settings, Factor XIIIa antibody staining is frequently used to characterize fibrohistiocytic lesions. Dermatofibroma typically demonstrates strong cytoplasmic staining, whereas dermatofibrosarcoma protuberans generally lacks expression, supporting its value in differential assessment. Expression patterns have also been described in inflammatory and reparative conditions where macrophage activation is present.

This clone has been validated using a human protein microarray platform containing thousands of full-length human proteins, demonstrating high specificity for the intended F13A1 target with minimal off-target reactivity. Protein microarray validation supports confidence in target selectivity when studying coagulation biology, stromal cell populations, and macrophage-associated processes. Factor XIIIa Antibody protein microarray validated clone F13A1/1683 is suitable for detecting Factor XIII A chain expression in relevant research applications, where staining is typically cytoplasmic in positive dendritic or macrophage-lineage cells.

Application Notes

Titering of the Factor XIIIa antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A human partial recombinant protein corresponding to amino acids 46-181 was used as the immunogen for the Factor XIIIa antibody-protein microarray validated clone F13A1/1683.

Storage

Store the Factor XIIIa antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).