Factor XIIIa Antibody - HuProt Validation [clone F13A1/1448] (V3499)

Description

Factor XIIIa Antibody - HuProt Validation clone F13A1/1448 recognizes Factor XIII A chain, the catalytic transglutaminase subunit encoded by the F13A1 gene on chromosome 6p25.1. Factor XIII A chain is a cytoplasmic enzyme expressed in platelets, macrophages, dermal dendritic cells, and select stromal cell populations. Following activation by thrombin and calcium, the inactive zymogen is cleaved to generate active Factor XIIIa, which stabilizes fibrin clots by catalyzing covalent cross-linking of fibrin monomers. This final step of the coagulation cascade is essential for clot strength and resistance to fibrinolysis.

Factor XIII A chain belongs to the transglutaminase family and contains a catalytic core domain that forms epsilon-gamma glutamyl-lysine isopeptide bonds between substrate proteins. In circulation, coagulation factor XIII exists as a heterotetramer composed of two A subunits and two B subunits. Upon activation, the B subunits dissociate and the A subunits become enzymatically active. Beyond its established role in hemostasis, Factor XIIIa contributes to extracellular matrix stabilization by cross-linking fibronectin, collagen, and other structural proteins, supporting wound healing and tissue remodeling.

In normal tissues, Factor XIII A chain is highly expressed in dermal dendrocytes and tissue macrophages. It is also detectable in placenta and bone marrow-derived cells. In fibrohistiocytic lesions, Factor XIIIa antibody staining is frequently used to characterize dermatofibroma, which typically shows strong cytoplasmic positivity, while dermatofibrosarcoma protuberans generally lacks expression. Expression has also been reported in inflammatory and reparative conditions involving macrophage activation and stromal remodeling.

This clone has been validated using the HuProt(TM) human protein microarray platform containing more than 19,000 full-length human proteins. HuProt validation demonstrates strong target selectivity for F13A1 with clear separation from unrelated proteins, supporting high specificity and minimal cross-reactivity. Factor XIIIa Antibody - HuProt validation clone F13A1/1448 is suitable for detecting Factor XIII A chain expression in relevant research applications, where staining is typically cytoplasmic in positive dendritic or macrophage-lineage cells.

Application Notes

Titering of the Factor XIIIa antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A human partial recombinant protein corresponding to amino acids 46-181 was used as the immunogen for the Factor XIIIa antibody with HuProt validation.

Storage

Store the Factor XIIIa antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).