FACL4 Antibody / Long Chain Acyl-CoA Synthetase 4 Antibody (RQ6920)

FACL4 Antibody Human, Rat and Mouse WB. Western blot analysis of human U-2 OS (lane 1), PC-3 (lane 2), HeLa (lane 3), Caco-2 (lane 4), HEL (lane 5), and U-87 MG (lane 6) cell lysates, rat brain (lane 7), rat lung (lane 8), rat stomach (lane 9), rat PC-12 (lane 10) lysates, and mouse brain (lane 11), mouse lung (lane 12), mouse stomach (lane 13), and mouse NIH 3T3 (lane 14) cell lysates using FACL4 Antibody / Long Chain Acyl-CoA Synthetase 4 Antibody. A predominant band is detected at approximately 74-80 kDa across the tested samples, corresponding to the expected molecular weights of the short and long FACL4 isoforms. The consistent detection in multiple human, rat, and mouse tissues and cell lines demonstrates broad cross-species reactivity. This FACL4 Antibody is well suited for Western blot analysis of long chain acyl-CoA synthetase 4 expression in studies of lipid metabolism, fatty acid activation, ferroptosis, and cellular energy homeostasis.

FACL4 Antibody Brain and Cancer Cell WB. Western blot analysis of human HepG2 (lane 1), PC-3 (lane 2), HeLa (lane 3), and Caco-2 (lane 4) cell lysates, rat brain (lane 5) and PC-12 (lane 6) lysates, and mouse brain (lane 7) and NIH/3T3 (lane 8) cell lysates using FACL4 Antibody / Long Chain Acyl-CoA Synthetase 4 Antibody. A specific band is detected at approximately 74 kDa in all tested samples, consistent with the expected molecular weight of the short FACL4 isoform. Although ACSL4 also has a long isoform of approximately 79-80 kDa, the shorter isoform predominates under these experimental conditions. This FACL4 Antibody is well suited for Western blot analysis of long chain acyl-CoA synthetase 4 expression in studies of lipid metabolism, fatty acid activation, ferroptosis, and cellular energy homeostasis.

FACL4 Antibody Human Cervical Cancer Cell IF. Immunofluorescent staining of SiHa cells using FACL4 Antibody / Long Chain Acyl-CoA Synthetase 4 Antibody following enzyme-mediated antigen retrieval. FACL4 was detected with a green fluorescent secondary antibody, while nuclei were counterstained with DAPI (blue). The staining demonstrates predominantly cytoplasmic localization with a punctate intracellular distribution, consistent with the association of FACL4 with intracellular membranes involved in fatty acid activation and lipid metabolism. This FACL4 Antibody is well suited for immunofluorescent analysis of long chain acyl-CoA synthetase 4 expression, ferroptosis, and lipid metabolic pathways in human cells.