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Home >> Antibodies >> FABP4 Antibody / Fatty Acid Binding Protein 4

FABP4 Antibody / Fatty Acid Binding Protein 4 [clone rFABP4/8536] (V5248)

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  Catalog No Formulation Size Price (USD)  
Image V5248-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5248-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5248SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human placental tissue with FABP4 antibody (clone rFABP4/8536) at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human placental tissue with FABP4 antibody (clone rFABP4/8536) at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free FABP4 antibody (clone rFABP4/8536) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rFABP4/8536
Purity Protein A/G affinity
UniProt P15090
Localization Cytoplasm, Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This FABP4 antibody is available for research use only.
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Description

Fatty acid-binding proteins, designated FABPs, are a family of homologous, cytoplasmic proteins that are expressed in a highly tissue-specific manner and play an integral role in the balance between lipid and carbohydrate metabolism. FABPs mediate fatty acid (FA) and/or hydrophobic ligand uptake, transport and targeting within their respective tissues. The mechanisms underlying these actions can give rise to both passive diffusional uptake and protein-mediated transmembrane transport of FAs. FABPs are expressed in adipocytes (A-FABP), brain (B-FABP), epidermis (E-FABP, also designated psoriasis-associated FABP or PA-FABP), muscle and heart (H-FABP, also designated mammary-derived growth inhibitor or MDGI), intestine (I-FABP), liver (L-FABP), myelin (M-FABP) and testis (T-FABP). The human A-FABP gene is organized into 4 exons, maps to chromosome 8q21.13, and encodes a 132 amino acid protein. A-FABP protein comprises approximately 1% of the total cytosolic protein in human adipose tissue.

Application Notes

Optimal dilution of the FABP4 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 1-132) from the human protein was used as the immunogen for the FABP4 antibody.

Storage

Aliquot the FABP4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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