FABP3 Antibody / Cardiac Energy Metabolism Marker [clone FABP3/3430] (V9473)

FABP3 Antibody Multi-Species Heart Tissue WB. Western blot analysis of human heart, mouse heart, rat heart, hamster heart, guinea pig heart, and rabbit heart tissue lysates using FABP3 Antibody clone FABP3/3430. A prominent band is detected at approximately 15 kDa in all tested cardiac tissue samples, consistent with the predicted molecular weight of Fatty acid-binding protein 3 / FABP3. The highly conserved cross-species cardiac expression pattern is consistent with the established role of FABP3 in intracellular fatty acid transport, myocardial lipid utilization, and oxidative energy metabolism within cardiomyocytes.

FABP3 Antibody FFEP Human Heart Tissue IHC. Immunohistochemistry analysis of FFPE human heart tissue stained with clone FABP3/3430. Cardiomyocytes exhibit diffuse cytoplasmic HRP-DAB brown staining for Fatty acid-binding protein 3 / FABP3, consistent with the known enrichment of this intracellular lipid transport protein in metabolically active cardiac muscle. The staining pattern supports the established role of FABP3 in myocardial fatty acid utilization and oxidative bioenergetics within cardiomyocytes. Hematoxylin counterstain highlights nuclei in blue. HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before testing.

FABP3 Antibody Multi-Tissue WB. Western blot analysis of human intestine, mouse intestine, rat intestine, human heart, mouse heart, rat heart, human skeletal muscle, mouse skeletal muscle, and rat skeletal muscle tissue lysates using FABP3 antibody clone FABP3/3430. A band is detected at approximately 15 kDa across all tested samples, consistent with the predicted molecular weight of Fatty acid-binding protein 3 / FABP3. Stronger signal intensity is observed in cardiac and skeletal muscle lysates, aligning with the known enrichment of FABP3 in highly oxidative tissues that rely on fatty acid transport and aerobic energy metabolism.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using FABP3 antibody (clone FABP3/3430). These results demonstrate the foremost specificity of the FABP3/3430 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

FABP3 Antibody Heart WB. Western blot analysis of human heart lysate using FABP3 Antibody. A dominant band is detected at approximately 14-15 kDa, consistent with the predicted molecular weight of Fatty acid-binding protein 3 / FABP3. A faint lower migrating band is also observed and may represent minor proteolytic processing or a faster-migrating low molecular weight species. The prominent cardiac signal aligns with the established enrichment of FABP3 in cardiomyocytes and its role in intracellular fatty acid transport and oxidative energy metabolism.

IHC staining of FFPE human kidney tissue with FABP3 antibody (clone FABP3/3430) HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.