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Home >> Antibodies >> FABP2 Antibody / Fatty acid binding protein 2

FABP2 Antibody / Fatty acid binding protein 2 [clone FABP2/6344] (V5886)

  Catalog No Formulation Size Price (USD)  
Image V5886-100UG 0.2 mg/ml in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 559
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V5886-20UG 0.2 mg/ml in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 259
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V5886SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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Western blot analysis of FABP2 in intestinal tissues. Western blot analysis was performed using tissue lysates from human intestine, mouse intestine, rat intestine, hamster intestine, guinea pig intestine, and rabbit intestine probed with FABP2/Fatty acid binding protein 2 antibody (clone FABP2/6344). A distinct immunoreactive band is observed at the predicted molecular weight of FABP2 across all intestinal samples, consistent with conserved expression of fatty acid binding protein 2 in absorptive intestinal epithelium. Predicted molecular weight ~15 kDa.
Immunohistochemistry of FABP2 in human small intestine. Formalin-fixed, paraffin-embedded human small intestine tissue stained with FABP2/Fatty acid binding protein 2 antibody (clone FABP2/6344). Brown chromogenic signal indicates FABP2-positive cytoplasmic staining in absorptive intestinal epithelial cells. Surrounding stromal and lamina propria cells show minimal to no staining. Tissue sections were subjected to heat-induced epitope retrieval using 10mM Tris with 1mM EDTA, pH 9.0, followed by incubation with the primary antibody.
SDS-PAGE Analysis of purified FABP2/Fatty acid binding protein 2 antibody (FABP2/6344). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing more than 19,000 full-length human proteins using FABP2/Fatty acid binding protein 2 antibody (FABP2/6344). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western blot analysis of human intestine, mouse intestine and rat intestine tissue lysates using FABP2/Fatty acid binding protein 2 antibody (FABP2/6344). Predicted molecular weight ~15 kDa.
Western blot analysis of human small intestine tissue lysates using FABP2/Fatty acid binding protein 2 antibody (FABP2/6344). Predicted molecular weight ~15 kDa.
Species Reactivity Guinea Pig, Hamster, Human, Mouse, Rabbit, Rat
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2, kappa
Clone Name FABP2/6344
UniProt P12104
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This FABP2/Fatty acid binding protein 2 antibody is available for research use only.
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Description

Fatty acid binding protein 2 is a small cytoplasmic lipid chaperone encoded by the FABP2 gene and is also widely referred to as intestinal fatty acid binding protein, I-FABP, or IFABP. FABP2 is a member of the fatty acid binding protein family, a group of low molecular weight intracellular proteins that bind long-chain fatty acids and other hydrophobic ligands to facilitate their intracellular trafficking. This FABP2 antibody targets Fatty acid binding protein 2, which is characteristically localized to the cytoplasm of absorptive epithelial cells in the small intestine, where it plays a central role in dietary lipid handling.

Fatty acid binding protein 2 is highly enriched in enterocytes of the jejunum and ileum, reflecting its specialized function in intestinal lipid absorption. By binding free fatty acids taken up from the intestinal lumen, FABP2 supports intracellular transport to sites of esterification, oxidation, or incorporation into lipoproteins. This places Fatty acid binding protein 2 at a critical junction between nutrient uptake and systemic lipid metabolism. As a member of the FABP family, FABP2 shares a conserved beta-barrel structure that forms a hydrophobic ligand-binding pocket, while maintaining tissue-specific expression patterns that distinguish it from other FABP isoforms.

Beyond its physiological role, Fatty acid binding protein 2 has gained significance as a biomarker in gastrointestinal research. Intestinal fatty acid binding protein is rapidly released into circulation following enterocyte injury, making FABP2 a useful indicator of intestinal epithelial damage in conditions such as ischemia, inflammatory bowel disease, and sepsis-associated gut barrier dysfunction. For this reason, FABP2 antibody reagents are frequently used to study intestinal integrity, epithelial turnover, and disease-associated alterations in lipid handling. The clone FABP2/6344 is designed to detect Fatty acid binding protein 2 expression in research applications, supporting studies of normal intestinal biology as well as pathological states involving epithelial injury or altered metabolism.

At the cellular level, Fatty acid binding protein 2 is predominantly cytoplasmic, consistent with its role in intracellular lipid transport. Expression is largely restricted to differentiated absorptive epithelial cells rather than crypt progenitors, aligning FABP2 expression with mature enterocyte function. This restricted expression profile contributes to the specificity of Fatty acid binding protein 2 as a marker of small intestinal epithelium and distinguishes it from other fatty acid binding proteins expressed in liver, adipose tissue, or immune cells.

Application Notes

Optimal dilution of the FABP2/Fatty acid binding protein 2 antibody should be determined by the researcher.

Immunogen

A recombinant fragment (around amino acids 1-132) of human FABP2 protein (exact sequence is proprietary) was used as the immunogen for the FABP2/Fatty acid binding protein 2 antibody.

Storage

FABP2/Fatty acid binding protein 2 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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