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Home >> Antibodies >> FABP1 Antibody for IHC / Fatty acid-binding protein 1

FABP1 Antibody for IHC / Fatty acid-binding protein 1 [clone MSVA-501M] (V6073)

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  Catalog No Formulation Size Price (USD)  
Image V6073-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 654.50
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V6073-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
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Immunohistochemistry of FABP1 Antibody for IHC (clone MSVA-501M) in human tissue microarrays. FFPE human normal and cancer tissues were stained with FABP1 antibody for IHC (clone MSVA-501M). In the normal tissue gallery, strong cytoplasmic HRP-DAB brown staining is observed in hepatocytes of liver, consistent with the known high expression of Liver fatty acid binding protein. Prominent cytoplasmic staining is also seen in small intestinal enterocytes and renal proximal tubular epithelial cells, while most other tissues show low to absent staining. In the cancer tissue gallery, hepatocellular carcinoma demonstrates retained cytoplasmic FABP1 expression, whereas many non-hepatic tumors show minimal or negative staining, consistent with reported expression patterns in Human Protein Atlas. The overall staining distribution supports the expected tissue-specific expression profile of FABP1.
Species Reactivity Human
Format Purified
Host Mouse
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2b, kappa
Clone Name MSVA-501M
UniProt P07148
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1:100-1:200
Limitations This FABP1/Fatty acid-binding protein 1 antibody is available for research use only.
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Description

FABP1 Antibody for IHC recognizes Fatty acid-binding protein 1, a small cytoplasmic lipid chaperone encoded by the FABP1 gene and commonly referred to as Liver fatty acid binding protein or L-FABP. FABP1 belongs to the fatty acid binding protein family, a group of highly conserved intracellular proteins that coordinate the uptake, transport, and metabolism of long chain fatty acids and other hydrophobic ligands. The human FABP1 gene is located on chromosome 2p11.2 and is predominantly expressed in hepatocytes, with additional expression in renal proximal tubular epithelium and small intestinal enterocytes.

Structurally, Fatty acid-binding protein 1 consists of a beta barrel domain that forms a hydrophobic binding pocket capable of accommodating long chain fatty acids, bile acids, and eicosanoids. By binding and solubilizing these ligands, FABP1 facilitates their intracellular trafficking to sites of beta oxidation, triglyceride synthesis, or signaling pathways. In the liver, FABP1 plays a central role in lipid homeostasis, energy metabolism, and protection against lipotoxic stress. Its high cytoplasmic abundance in hepatocytes reflects its importance in hepatic fatty acid handling.

In normal tissues, FABP1 expression is strongest in liver, where diffuse cytoplasmic staining in hepatocytes is expected. In kidney, cytoplasmic expression is typically observed in proximal tubular epithelial cells, consistent with a role in fatty acid reabsorption and metabolism. Intestinal epithelial cells of the small intestine also demonstrate cytoplasmic expression. Because of this characteristic distribution, FABP1 antibody is widely used in research to assess hepatocellular differentiation and to study metabolic adaptations in liver and kidney tissues.

Altered expression of Fatty acid-binding protein 1 has been associated with metabolic disorders, nonalcoholic fatty liver disease, and hepatocellular carcinoma. Changes in FABP1 levels may reflect shifts in lipid flux, oxidative stress, and cellular metabolic state. A mouse monoclonal antibody such as clone MSVA-501M provides a consistent tool for detecting FABP1 in formalin-fixed, paraffin-embedded tissues. FABP1 Antibody for IHC is suitable for detecting FABP1 expression in research settings focused on hepatic biology, renal tubular function, and lipid metabolism.

Application Notes

1. Optimal dilution of the FABP1/Fatty acid-binding protein 1 antibody should be determined by the researcher.

2. This FABP1/Fatty acid-binding protein 1 antibody is recombinantly produced by expression in human HEK293 cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Immunogen

Human recombinant FABP1 protein fragment (around amino acids 1-127) (exact sequence is proprietary) was used as the immunogen for the FABP1/Fatty acid-binding protein 1 antibody for IHC.

Storage

FABP1/Fatty acid-binding protein 1 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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