FABP Antibody Rabbit Polyclonal (Liver form) (R31902)

Western blot analysis of FABP antibody in rodent tissues. Rat liver (lane 1), rat intestine (lane 2), rat RH35 cells (lane 3), rat PC-12 cells (lane 4), mouse liver (lane 5), and mouse small intestine (lane 6) lysates were analyzed using FABP antibody rabbit polyclonal. The predicted molecular weight of Liver fatty acid binding protein is approximately 14 kDa. A strong band is observed near 14 kDa in rat and mouse liver samples, consistent with high FABP1 expression in hepatocytes. Clear bands are also detected in rat and mouse intestinal tissues, reflecting known expression in enterocytes. Signal in RH35 cells is present and consistent with hepatic origin, while PC-12 cells show minimal to low expression. The observed band pattern aligns with the expected tissue distribution of FABP1.

Immunohistochemistry of FABP antibody in human intestinal cancer tissue. FFPE human intestinal carcinoma was stained with FABP antibody rabbit polyclonal. Predominant cytoplasmic HRP-DAB brown staining is observed in gland-forming tumor epithelial cells, consistent with Liver fatty acid binding protein expression in intestinal epithelium. Stromal cells show minimal staining. Heat induced epitope retrieval was performed by boiling paraffin sections in pH 6, 10 mM citrate buffer for 20 minutes followed by cooling prior to antibody incubation.

IHC testing of FFPE mouse intestine with FABP antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE rat intestine with FABP antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

Western blot testing of 1) human HCCT, 2) monkey liver and 3) chicken liver tissue lysate with FABP antibody. Predicted molecular weight: ~14 kDa.