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Home >> Antibodies >> F13A1 Antibody for IHC / Coagulation factor XIII A chain

F13A1 Antibody for IHC / Coagulation factor XIII A chain [clone MSVA-813R] (V6072)

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  Catalog No Formulation Size Price (USD)  
Image V6072-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 654.50
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V6072-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
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Immunohistochemistry of F13A1 Antibody for IHC in human tissue microarrays. FFPE human normal and cancer tissues were stained with Coagulation factor XIII A chain rabbit recombinant monoclonal antibody clone MSVA-813R. Predominant cytoplasmic HRP-DAB brown staining is observed in scattered stromal and histiocytic cells, consistent with known Factor XIII A chain expression in dermal dendrocytes and macrophage-lineage cells. In normal tissues, positive cells are evident within skin, lymph node, and placenta, while most epithelial compartments show minimal staining. In the cancer tissue gallery, cytoplasmic staining is present in subsets of fibrohistiocytic and macrophage-rich tumors, whereas many epithelial malignancies demonstrate absent or very limited labeling. The staining pattern and tissue distribution align with reported expression data from the Human Protein Atlas.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-813R
UniProt P00488
Localization Cytoplasm, Secreted
Applications Immunohistochemistry (FFPE) : 1:100-1:200
Limitations This F13A1/Coagulation factor XIII A chain antibody is available for research use only.
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Description

F13A1 Antibody for IHC recognizes Coagulation factor XIII A, the catalytic transglutaminase subunit encoded by the F13A1 gene on chromosome 6p25.1. Coagulation factor XIII A, also referred to as Factor XIIIa or Factor XIII A chain, is a cytoplasmic enzyme expressed in platelets, macrophages, dermal dendritic cells, and selected stromal populations. Upon activation by thrombin and calcium, the inactive zymogen is cleaved to generate active Factor XIIIa, which catalyzes covalent cross-linking of fibrin monomers. This reaction stabilizes the fibrin clot and confers resistance to fibrinolysis, representing the final enzymatic step of the coagulation cascade.

Coagulation factor XIII A belongs to the transglutaminase family and contains a catalytic core domain that mediates formation of epsilon-gamma glutamyl-lysine isopeptide bonds between substrate proteins. In plasma, factor XIII circulates as a heterotetramer composed of two catalytic A subunits and two non-catalytic B subunits. Following thrombin-mediated activation, the B subunits dissociate and the A subunits become enzymatically active. Beyond clot stabilization, Factor XIIIa contributes to extracellular matrix remodeling by cross-linking fibronectin, collagen, and other structural proteins, supporting wound healing and tissue repair processes.

In normal tissues, Coagulation factor XIII A expression is prominent in dermal dendrocytes and tissue macrophages. It is also detectable in placenta and bone marrow-derived cells. In fibrohistiocytic lesions, F13A1 Antibody for IHC is frequently applied in research settings to help characterize dermatofibroma, which typically demonstrates strong cytoplasmic positivity in spindle-shaped dermal cells, whereas dermatofibrosarcoma protuberans generally lacks staining. Expression patterns have also been described in inflammatory and reparative conditions involving macrophage activation and stromal remodeling.

Inherited deficiency of Factor XIII results in impaired clot stability and delayed wound healing, underscoring the biologic importance of F13A1 function. Altered expression has been observed in inflammatory and fibrotic disorders, reflecting its broader role in immune cell biology and extracellular matrix stabilization. Clone MSVA-813R is a rabbit monoclonal antibody suitable for detecting Coagulation factor XIII A expression in immunohistochemistry and related research applications, where staining is typically cytoplasmic in dendritic or macrophage-lineage cells.

Application Notes

1. Optimal dilution of the F13A1/Coagulation factor XIII A chain antibody should be determined by the researcher.

2. This F13A1/Coagulation factor XIII A chain antibody is recombinantly produced by expression in human HEK293 cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Immunogen

A recombinant fragment of human Factor XIIIa protein (aa46-181) (exact sequence is proprietary) was used as the immuongen for the F13A1/Coagulation factor XIII A chain antibody for IHC.

Storage

F13A1/Coagulation factor XIII A chain antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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