EXOG Antibody / Nuclease EXOG, mitochondrial Antibody (FY13628)

EXOG Antibody by IHC in human endometrial carcinoma. Immunohistochemistry of paraffin-embedded human endometrial carcinoma using Nuclease EXOG, mitochondrial Antibody shows prominent brown staining in glandular epithelial tumor structures with hematoxylin-counterstained nuclei. Heat-mediated antigen retrieval was performed using EDTA buffer at pH 8.0. Sections were blocked with goat serum and incubated with 2 ug/mL primary antibody overnight at 4oC, followed by HRP-conjugated secondary antibody and DAB visualization.

EXOG Antibody by IF in HeLa cells. Immunofluorescence analysis using Nuclease EXOG, mitochondrial Antibody reveals green fluorescence in nuclear and perinuclear regions, with visible nuclear enrichment in HeLa cells. Red alpha-tubulin counterstaining highlights the cytoskeleton. Cells were treated with enzyme-based antigen retrieval and incubated with 5 ug/mL primary antibody overnight at 4oC before fluorescent secondary antibody detection. The image depicts the observed staining pattern without establishing organelle-specific colocalization.

EXOG Antibody by WB in human cell lines. Western blot analysis using Nuclease EXOG, mitochondrial Antibody detects a prominent immunoreactive band consistent with the expected molecular weight of approximately 41 kDa. Lanes 1-4 contain HeLa, HepG2, Jurkat and U251 whole-cell lysates, respectively. Reduced samples containing 30 ug total protein per lane were resolved by 10% SDS-PAGE and transferred to nitrocellulose. The membrane was blocked with 5% nonfat milk, incubated with 0.5 ug/mL primary antibody overnight at 4oC, and probed with an HRP-conjugated secondary antibody. Signal was visualized by chemiluminescence.