EXOC7 Antibody / Exocyst complex component 7 Antibody (FY13602)

EXOC7 Antibody by FACS in RT4 cells. Flow cytometry of fixed, permeabilized RT4 cells stained with Exocyst complex component 7 Antibody shows a rightward fluorescence shift (blue) relative to the rabbit IgG isotype control (green) and unstained control (red). Cells were fixed in 4% paraformaldehyde, stained with 1 ug antibody per 1 x 10^6 cells, and detected with a fluorophore-conjugated secondary antibody. The experiment evaluates intracellular immunoreactivity, not cell-surface expression.

EXOC7 Antibody by IHC in human breast carcinoma. Immunohistochemistry of paraffin-embedded human breast carcinoma using Exocyst complex component 7 Antibody reveals brown cytoplasmic staining in tumor epithelial cells. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), blocking with goat serum, and primary antibody incubation at 2 ug/mL overnight at 4oC. Bound antibody was detected using an HRP-conjugated secondary antibody and DAB chromogen.

EXOC7 Antibody by WB in human and rodent samples. Western blot analysis using Exocyst complex component 7 Antibody detects a prominent immunoreactive band near 75 kDa in human 293T (lane 1), HeLa (lane 2), RT4 (lane 3), rat brain (lane 4), rat C6 (lane 5), mouse brain (lane 6), and NIH/3T3 (lane 7). The observed migration is compared with the calculated molecular weight of approximately 83 kDa. Reduced lysates containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, the membrane was incubated with primary antibody at 0.5 ug/mL overnight at 4oC, followed by HRP-conjugated secondary antibody and chemiluminescent detection.