EXOC2 Antibody / Exocyst complex component 2 Antibody (FY13571)

EXOC2 Antibody by IF in A549 cells. Immunofluorescence using Exocyst complex component 2 Antibody demonstrates a predominantly perinuclear and nuclear-associated green signal in A549 cells against extensive red cytoplasmic counterstaining. The image shows signal concentrated in the central cell regions, although this single figure does not establish precise subcellular compartment identity. The merged fluorescence channels show individual cells and their outlines, permitting comparison of EXOC2-associated staining with the cytoplasmic counterstain.

EXOC2 Antibody by WB in human and rodent cell and tissue lysates. Western blot analysis using Exocyst complex component 2 Antibody detects a prominent immunoreactive band near 100 kDa in human RT4 (lane 1), SH-SY5Y (lane 2), rat brain (lane 3), mouse brain (lane 4), Neuro-2a (lane 5), and NIH/3T3 (lane 6). The calculated molecular weight is approximately 104.1 kDa. Samples were analyzed under reducing conditions with 30 ug protein per lane using 8% SDS-PAGE, followed by transfer to nitrocellulose. Membranes were blocked with 5% nonfat milk and incubated with primary antibody at 0.5 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and chemiluminescent substrate were used for visualization.

EXOC2 Antibody by IHC in human brain tissue. Immunohistochemistry of paraffin-embedded human brain tissue using Exocyst complex component 2 Antibody demonstrates brown staining in scattered neural cells, with stronger labeling in several cell bodies against lightly stained neuropil, with hematoxylin-counterstained nuclei appearing blue. Sections were subjected to heat-mediated antigen retrieval in EDTA buffer at pH 8.0 and blocked with 10% goat serum. The primary antibody was incubated at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB detection.