EWSR1 Antibody / RNA Binding Protein Antibody (R32181)

EWSR1 Antibody Human Thyroid Cancer IHC. Immunohistochemical analysis of FFPE human thyroid cancer tissue following heat-induced epitope retrieval in pH8 EDTA buffer. Tissue sections were incubated with EWSR1 Antibody at 2 ug/ml overnight, followed by an HRP-conjugated secondary antibody and DAB chromogen for detection. Strong predominantly nuclear staining is observed throughout the neoplastic epithelial cells, consistent with the established nuclear localization of EWSR1 in transcriptional regulation and RNA processing. This staining pattern demonstrates that this EWSR1 Antibody, an RNA Binding Protein Antibody, is suitable for detecting endogenous EWSR1 expression in human FFPE thyroid cancer tissue by immunohistochemistry.

EWSR1 Antibody Human Testis Cancer IHC. Immunohistochemical analysis of FFPE human testis cancer tissue following heat-induced epitope retrieval in pH8 EDTA buffer. Tissue sections were incubated with EWSR1 Antibody at 2 ug/ml overnight, followed by an HRP-conjugated secondary antibody and DAB chromogen for visualization. Distinct nuclear staining is observed in the majority of tumor cells, consistent with the predominantly nuclear localization of EWSR1 as an RNA-binding protein involved in transcriptional regulation and RNA processing. This staining pattern demonstrates that this EWSR1 Antibody, an RNA Binding Protein Antibody, is suitable for detecting endogenous EWSR1 expression in human FFPE tissue by immunohistochemistry.

EWSR1 Antibody Rat Spleen IHC. Immunohistochemical analysis of FFPE rat spleen tissue following heat-induced epitope retrieval in pH8 EDTA buffer. Tissue sections were incubated with EWSR1 Antibody at 2 ug/ml overnight, followed by an HRP-conjugated secondary antibody and DAB chromogen for visualization. Moderate nuclear staining is observed throughout splenic cells, consistent with the widespread expression and predominantly nuclear localization of EWSR1 in transcriptional regulation and RNA processing. This staining pattern demonstrates that this EWSR1 Antibody, an RNA Binding Protein Antibody, is suitable for detecting endogenous EWSR1 in rat FFPE tissue by immunohistochemistry.

EWSR1 Antibody HeLa Cell IP. Immunoprecipitation of endogenous EWSR1 from HeLa whole cell lysate was performed using EWSR1 Antibody, followed by Western blot detection with the same antibody. Lane 1: HeLa input lysate (30 ug). Lane 2: rabbit IgG immunoprecipitation negative control. Lane 3: EWSR1 immunoprecipitated from 500 ug of HeLa lysate using 2 ug of EWSR1 Antibody. A distinct band is detected at approximately 90-95 kDa in the input and EWSR1 immunoprecipitation lanes, while no corresponding signal is observed in the IgG control. Although the predicted molecular weight of EWSR1 is approximately 69 kDa, the protein commonly migrates at a higher apparent molecular weight due to its intrinsically disordered regions. These results demonstrate that this EWSR1 Antibody, an RNA Binding Protein Antibody, efficiently immunoprecipitates endogenous EWSR1 for downstream protein interaction and molecular biology studies.

EWSR1 Antibody Human, Mouse and Rat Cell WB. Western blot analysis of EWSR1 was performed using lysates from human Jurkat, K562, HeLa, and RT4 cells, rat testis and C6 cells, and mouse testis. A prominent immunoreactive band is detected at approximately 90-95 kDa in all samples, with additional lower molecular weight bands visible in several lanes, particularly the testis lysates. Although the predicted molecular weight of EWSR1 is approximately 69 kDa, EWSR1 commonly migrates at a higher apparent molecular weight due to its amino acid composition and extensive intrinsically disordered regions, which alter electrophoretic mobility. These results demonstrate that this EWSR1 Antibody, an RNA Binding Protein Antibody, consistently detects endogenous EWSR1 across multiple human and rodent cell and tissue lysates by Western blot.

EWSR1 Antibody HeLa Cell IF. Immunofluorescence analysis of HeLa cells following enzymatic antigen retrieval. Cells were incubated with EWSR1 Antibody at 5 ug/ml together with a Tubulin Alpha antibody, followed by fluorophore-conjugated secondary antibodies. EWSR1 is shown in green and Tubulin Alpha in red. EWSR1 staining is observed predominantly within the nucleus, with weaker diffuse cytoplasmic signal, consistent with its established role as an RNA-binding protein involved in transcriptional regulation and RNA processing. This localization demonstrates that this EWSR1 Antibody, an RNA Binding Protein Antibody, is suitable for visualizing endogenous EWSR1 expression and subcellular distribution in cultured cells by immunofluorescence.

EWSR1 Antibody RT4 Cell FACS. Flow cytometric analysis of fixed and permeabilized RT4 cells using EWSR1 Antibody at 1 ug/10^6 cells, followed by a Fluoro488-conjugated secondary antibody. The blue histogram represents EWSR1 staining, the green histogram represents the rabbit IgG isotype control, and the red histogram represents the unstained blank control. A pronounced rightward shift of the EWSR1-stained population relative to both controls demonstrates specific intracellular detection of endogenous EWSR1. These results show that this EWSR1 Antibody, an RNA Binding Protein Antibody, is suitable for flow cytometric analysis of EWSR1 expression in cultured cells.