EVPL Antibody / Envoplakin Antibody (FY13617)

EVPL Antibody by IHC in human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded human skin using Envoplakin Antibody shows prominent brown staining in epidermal keratinocytes, particularly within stratified epithelial layers. Antigen retrieval was performed with EDTA buffer at pH 8.0. Sections were blocked with 10% goat serum and incubated with primary antibody at 2 ug/mL overnight at 4oC. Detection used an HRP-conjugated secondary antibody and DAB chromogen, with hematoxylin counterstaining.

EVPL Antibody by IF in A431 cells. Immunofluorescence staining using Envoplakin Antibody shows green fluorescence predominantly along cell boundaries and within the peripheral cytoplasm, with DAPI-stained nuclei in blue. Cells were treated with enzymatic antigen retrieval reagent for 15 minutes, blocked with 10% goat serum, and incubated with primary antibody at 5 ug/mL overnight at 4oC. Fluoro488-conjugated goat anti-rabbit IgG was applied at 1:500 for 30 minutes before fluorescence microscopy.

EVPL Antibody by WB in human cell lines and mouse lung. Western blot analysis using Envoplakin Antibody detects an immunoreactive band consistent with the expected molecular weight of approximately 232 kDa. Lane assignments: human A431 (lane 1), HeLa (lane 2), RT4 (lane 3), HaCaT (lane 4), and mouse lung (lane 5). Each lane contained 30 ug total protein under reducing conditions, resolved by 8% SDS-PAGE and transferred to nitrocellulose. Membranes were blocked with 5% nonfat milk and incubated with primary antibody at 0.5 ug/mL overnight at 4oC. An HRP-conjugated secondary antibody and chemiluminescent substrate were used for detection.

EVPL Antibody by IHC in human cervical carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human cervical carcinoma using Envoplakin Antibody shows prominent membranous and cytoplasmic brown staining in epithelial tumor cells. Antigen retrieval was performed with EDTA buffer at pH 8.0. Sections were blocked with 10% goat serum and incubated with primary antibody at 2 ug/mL overnight at 4oC. Detection used an HRP-conjugated secondary antibody and DAB chromogen, with hematoxylin counterstaining.

EVPL Antibody by IHC in rat lung. Immunohistochemistry of formalin-fixed, paraffin-embedded rat lung using Envoplakin Antibody shows brown staining in bronchiolar epithelial cells, with less prominent signal in surrounding lung parenchyma. Antigen retrieval was performed with EDTA buffer at pH 8.0. Sections were blocked with 10% goat serum and incubated with primary antibody at 2 ug/mL overnight at 4oC. Detection used an HRP-conjugated secondary antibody and DAB chromogen, with hematoxylin counterstaining.