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Home >> Antibodies >> Eukaryotic translation initiation factor 5 Antibody / EIF5

Eukaryotic translation initiation factor 5 Antibody / EIF5 (RQ8103)

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  Catalog No Formulation Size Price (USD)  
Image RQ8103 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human esophageal squamous carcinoma tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human esophageal squamous carcinoma tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cancer tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric cancer tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric cancer tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Eukaryotic translation initiation factor 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) K562, 2) HeLa, 3) HepG2, 4) PC-3 and 5) Caco-2 cell lysate with Eukaryotic translation initiation factor 5 antibody. Predicted molecular weight ~49 kDa.
Flow cytometry testing of human K562 cells with Eukaryotic translation initiation factor 5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Eukaryotic translation initiation factor 5 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P55010
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Eukaryotic translation initiation factor 5 antibody is available for research use only.
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Description

Eukaryotic translation initiation factor 5 is a protein that in humans is encoded by the EIF5 gene. Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations.

Application Notes

Optimal dilution of the Eukaryotic translation initiation factor 5 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids D11-D423) was used as the immunogen for the Eukaryotic translation initiation factor 5 antibody.

Storage

After reconstitution, the Eukaryotic translation initiation factor 5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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