- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
ETV2 Antibody / ETS variant transcription factor 2 (FY13334)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY13334 | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml | 100 ug | 439 |
ETV2 antibody detects ETS variant transcription factor 2, a nuclear DNA-binding protein encoded by the ETV2 gene on chromosome 19p13.3. ETV2 belongs to the ETS family of transcription factors and is an essential regulator of vascular and hematopoietic development. It is highly expressed in endothelial progenitor cells, embryonic mesoderm, and early hematopoietic lineages, where it drives the transcription of genes required for blood vessel and blood cell formation. ETV2 functions as a master transcriptional activator that initiates endothelial lineage commitment during embryogenesis.
ETV2 directly binds to ETS consensus sequences in the promoters of endothelial genes, activating transcription of critical targets such as VEGFR2 (KDR), TIE2, and CDH5 (VE-cadherin). This activity positions ETV2 at the top of the transcriptional hierarchy controlling vasculogenesis and angiogenesis. It cooperates with transcription factors such as GATA2 and FOXC2 to orchestrate endothelial differentiation and vascular remodeling. ETV2 also transiently regulates hematopoietic gene expression, linking early vascular and blood development.
Structurally, ETV2 contains an ETS DNA-binding domain responsible for recognizing GGAA/T motifs in target promoters and a transactivation domain that recruits coactivators such as CBP/p300. These interactions enable the chromatin remodeling necessary for endothelial gene induction. ETV2 belongs to the ETS family of transcription factors, which share highly conserved DNA-binding domains that control diverse developmental and cellular processes.
Functionally, ETV2 plays a crucial role in embryonic vasculogenesis, angiogenesis, and reprogramming of somatic cells toward endothelial fates. Forced expression of ETV2 in fibroblasts can induce endothelial gene expression, demonstrating its potency as a lineage reprogramming factor. ETV2 also participates in VEGF and MAPK signaling pathways, coordinating growth factor responses during vascular morphogenesis. Co-localization studies have shown ETV2 associating with GATA2 and TAL1 in the nucleus, forming transcriptional complexes essential for endothelial specification.
Dysregulation of ETV2 expression disrupts vascular and hematopoietic development and has been implicated in congenital heart defects and embryonic lethality in knockout models. In adult tissues, ETV2 expression is normally repressed, but its reactivation can promote angiogenesis in ischemic and regenerative contexts. Pathway involvement includes VEGF signaling, Notch signaling, and angiogenic transcriptional networks that coordinate vessel growth and stabilization.
Immunohistochemical staining using ETV2 antibody reveals strong nuclear localization in vascular endothelial cells and embryonic tissues. ETV2 antibody from NSJ Bioreagents is an excellent reagent for research in endothelial cell differentiation, vascular biology, and transcriptional regulation of angiogenesis.
Optimal dilution of the ETV2 antibody should be determined by the researcher.
E.coli-derived mouse ETV2 recombinant protein (Position: M1-Q335) was used as the immunogen for the ETV2 antibody.
After reconstitution, the ETV2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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