ETV1 Antibody / ETS translocation variant 1 Antibody (FY13555)

ETV1 Antibody by WB in human cell lines. Western blot analysis using ETS translocation variant 1 Antibody detects a band near 45 kDa in human PC-3 (lane 1), K562 (lane 2), SH-SY5Y (lane 3) and HeLa (lane 4) whole-cell lysates. The observed band migrates near 45 kDa, below the approximately 55 kDa calculated molecular weight of canonical ETV1. Alternative ETV1 isoforms and N-terminally truncated proteins have been documented in published studies, although the detected band has not been confirmed as a specific isoform. Reduced lysates containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. Membranes were blocked with 5% nonfat milk and incubated with primary antibody at 0.5 ug/mL overnight at 4oC. Bound antibody was visualized using HRP-conjugated secondary antibody and chemiluminescence.