ETS2 Antibody / Transcriptional Regulation and MAPK Signaling Marker Antibody [clone PCRP-ETS2-1D9] (V8921)

ETS2 Antibody Microarray Specificity Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ETS2 antibody (clone PCRP-ETS2-1D9). These results demonstrate the foremost specificity of the PCRP-ETS2-1D9 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

ETS2 Antibody HeLa IF. Immunofluorescence analysis of PFA-fixed human HeLa cells using ETS2 antibody (green) shows nuclear localization consistent with its role as a transcriptional regulator downstream of MAPK signaling, while phalloidin (red) highlights the actin cytoskeleton; detection was performed with clone PCRP-ETS2-1D9.

ETS2 Antibody HeLa FACS. Flow cytometry analysis of PFA-fixed human HeLa cells using ETS2 antibody shows a clear rightward shift in fluorescence intensity compared to the isotype control, consistent with intracellular detection of ETS2, a transcription factor involved in MAPK signaling; blue histogram represents ETS2 antibody staining and red histogram represents isotype control, with detection performed using clone PCRP-ETS2-1D9.

SDS-PAGE analysis of purified, BSA-free ETS2 antibody (clone PCRP-ETS2-1D9) as confirmation of integrity and purity.