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Home >> Antibodies >> ETS Variant 5 Antibody / ETV5

ETS Variant 5 Antibody / ETV5 (RQ5812)

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  Catalog No Formulation Size Price (USD)  
Image RQ5812 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human MCF7 cells with ETS Variant 5 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human MCF7 cells with ETS Variant 5 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) A549, 2) SW620, 3) HepG2, 4) rat C6, 5) mouse Neuro-2a and 6) mouse HEPA1-6 lysate with ETS Variant 5 antibody. Expected molecular weight: 58-70 kDa, doublet may be observed.
Flow cytometry testing of human HL-60 cells with ETS Variant 5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ETS Variant 5 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P41161
Localization Nuclear
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This ETS Variant 5 antibody is available for research use only.
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Description

Ets variant 5 (ETV5) (also named ERM transcription factor) is a transcription factor that in humans is encoded by the ETV5 gene. It is generated in Sertoli cells, which are found in the testes and play a crucial role in spermatogenesis. Its ortholog has been linked to both obesity and bipolar disorder.

Application Notes

Optimal dilution of the ETS Variant 5 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids D72-Q246) was used as the immunogen for the ETS Variant 5 antibody.

Storage

After reconstitution, the ETS Variant 5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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