ETNK1 Antibody / Ethanolamine kinase 1 Antibody (FY13632)

ETNK1 Antibody by IF in A549 cells. Immunofluorescence analysis of A549 cells using Ethanolamine kinase 1 Antibody reveals green predominantly cytoplasmic staining surrounding blue DAPI-labeled nuclei. Cells were treated for antigen retrieval, blocked with goat serum, and incubated with 5 ug/mL primary antibody overnight at 4oC. Bound antibody was detected using a fluorescent anti-rabbit secondary antibody.

ETNK1 Antibody by IHC in human ovarian carcinoma. Immunohistochemistry of paraffin-embedded human ovarian carcinoma using Ethanolamine kinase 1 Antibody demonstrates brown DAB staining in malignant epithelial cells with blue nuclear counterstaining. Heat-mediated antigen retrieval in EDTA buffer at pH 8.0 preceded incubation with 2 ug/mL primary antibody overnight at 4oC and HRP-based visualization.

ETNK1 Antibody by FACS in A549 cells. Intracellular flow cytometry of fixed, permeabilized A549 cells using Ethanolamine kinase 1 Antibody shows a rightward fluorescence shift for antibody-stained cells (blue) relative to rabbit IgG isotype control (green) and unstained blank control (red). The experiment measures intracellular antigen staining rather than cell-surface binding.

ETNK1 Antibody by FACS in MCF-7 cells. Intracellular flow cytometry of fixed, permeabilized MCF-7 cells using Ethanolamine kinase 1 Antibody shows a rightward shift in fluorescence for antibody-stained cells (blue) relative to the rabbit IgG isotype control (green) and unstained control (red). Cells were fixed with paraformaldehyde before staining.

ETNK1 Antibody by WB in human A549 (lane 1), HepG2 (lane 2), and MCF-7 (lane 3) whole-cell lysates. Western blot analysis using Ethanolamine kinase 1 Antibody detects an immunoreactive band consistent with the expected molecular weight of approximately 51 kDa. Samples were prepared under reducing conditions, with 30 ug of total protein per lane, and resolved by 10% SDS-PAGE. The lanes contain human A549 (lane 1), HepG2 (lane 2), and MCF-7 (lane 3) whole-cell lysates. Proteins were transferred to nitrocellulose, blocked with 5% nonfat milk, and incubated with primary antibody at 0.5 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and chemiluminescence were used for detection.