ETHE1 Antibody / Persulfide dioxygenase ETHE1, mitochondrial Antibody (FY13574)

ETHE1 Antibody by IF in U2OS cells. Immunofluorescence using Persulfide dioxygenase ETHE1, mitochondrial Antibody shows green cytoplasmic staining in U2OS cells, with signal extending around the blue DAPI-stained nuclei and appearing somewhat granular in the surrounding cytoplasm. This distribution is compatible with a mitochondrial protein, but the organelle identity is not directly confirmed by a colocalization marker in this figure. Following enzyme-mediated antigen retrieval and blocking with 10% goat serum, cells were incubated with primary antibody at 5 ug/mL overnight at 4oC and detected with a fluorescent goat anti-rabbit secondary antibody.

ETHE1 Antibody by IHC in human stomach tissue. Immunohistochemistry of paraffin-embedded human stomach tissue using Persulfide dioxygenase ETHE1, mitochondrial Antibody demonstrates brown staining concentrated in gastric glandular epithelial cytoplasm, including cells surrounding mucous vacuoles, with hematoxylin-counterstained nuclei appearing blue. Sections were subjected to heat-mediated antigen retrieval in EDTA buffer at pH 8.0 and blocked with 10% goat serum. The primary antibody was incubated at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB detection.

ETHE1 Antibody by WB in human cell lines and rodent tissues. Western blot analysis using Persulfide dioxygenase ETHE1, mitochondrial Antibody detects a prominent immunoreactive band near 28 kDa in HeLa (lane 1), HepG2 (lane 2), Jurkat (lane 3), RT4 (lane 4), rat liver (lane 5), rat stomach (lane 6), mouse liver (lane 7), and mouse stomach (lane 8). The calculated molecular weight is approximately 27.9 kDa. Samples were analyzed under reducing conditions with 30 ug protein per lane using 12% SDS-PAGE, followed by transfer to nitrocellulose. Membranes were blocked with 5% nonfat milk and incubated with primary antibody at 0.5 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and chemiluminescent substrate were used for visualization.

ETHE1 Antibody by IHC in human colon tissue. Immunohistochemistry of paraffin-embedded human colon tissue using Persulfide dioxygenase ETHE1, mitochondrial Antibody demonstrates brown cytoplasmic staining along colonic glandular epithelial cells, particularly the basal and lateral regions around mucin-rich goblet cells, with hematoxylin-counterstained nuclei appearing blue. Sections were subjected to heat-mediated antigen retrieval in EDTA buffer at pH 8.0 and blocked with 10% goat serum. The primary antibody was incubated at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB detection.

ETHE1 Antibody by IHC in human colon carcinoma. Immunohistochemistry of paraffin-embedded human colon carcinoma using Persulfide dioxygenase ETHE1, mitochondrial Antibody demonstrates dense brown cytoplasmic staining in malignant gland-forming epithelial cells, with hematoxylin-counterstained nuclei appearing blue. Sections were subjected to heat-mediated antigen retrieval in EDTA buffer at pH 8.0 and blocked with 10% goat serum. The primary antibody was incubated at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB detection.

ETHE1 Antibody by IHC in human stomach carcinoma. Immunohistochemistry of paraffin-embedded human stomach carcinoma using Persulfide dioxygenase ETHE1, mitochondrial Antibody demonstrates strong granular brown cytoplasmic staining in tumor epithelium surrounding glandular lumina, with hematoxylin-counterstained nuclei appearing blue. Sections were subjected to heat-mediated antigen retrieval in EDTA buffer at pH 8.0 and blocked with 10% goat serum. The primary antibody was incubated at 2 ug/mL overnight at 4oC, followed by peroxidase-conjugated secondary antibody and DAB detection.

ETHE1 Antibody by IP in human RT4 cells. Immunoprecipitation using Persulfide dioxygenase ETHE1, mitochondrial Antibody was assessed by Western blot of RT4 whole-cell lysate. Lane 1 contains input lysate, lane 2 is the rabbit IgG immunoprecipitation control, and lane 3 contains material precipitated with ETHE1 antibody. A band near 26-28 kDa is visible in the input and antibody immunoprecipitate, consistent with the approximately 28 kDa expected protein, while a prominent band near 50 kDa in the immunoprecipitate corresponds to immunoglobulin heavy chain. The experiment supports recovery of an ETHE1-reactive protein from RT4 lysate.