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Home >> Antibodies >> Estrogen Receptor beta Antibody

Estrogen Receptor beta Antibody [clone ERb455] (V2115)

  Catalog No Formulation Size Price (USD)  
Image V2115-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2115-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2115SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V2115IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC testing of human breast cancer stained with Estrogen Receptor beta antibody (ERb455).
IHC testing of human ovarian cancer stained with Estrogen Receptor beta antibody (ERb455).
FACS testing of human MCF-7 cells: Black=cells alone; Green=isotype control; Red=Estrogen Receptor beta antibody PE conjugate
FACS testing of human BT474 cells: Black=cells alone; Gray=isotype control; Green=Estrogen Receptor beta antibody AF488 conjugate
IHC testing of human gastric carcinoma stained with Estrogen Receptor beta antibody (ERb455).
IHC testing of human bladder carcinoma stained with Estrogen Receptor beta antibody (ERb455).
Western blot testing of human MCF7 cell lysate with Estrogen Receptor beta antibody (clone ERb455). Expected molecular weight: 53-59 kDa.
SDS-PAGE analysis of purified, BSA-free Estrogen Receptor beta antibody (clone ERb455) as confirmation of integrity and purity.
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name ERb455
Purity Protein G purified monoclonal antibody
Gene ID 2100
Localization Predominantly nuclear
Applications Western blot : 1-2ug/ml
Flow cytometry : 1-2ug/10^6 cells
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Estrogen Receptor beta antibody is available for research use only.
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Description

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including alpha and beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER alpha and beta are differentially activated by various ligands. Ligand interaction triggers a cascade of events, including dissociation from heat shock proteins, recepter dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER alpha and beta may be regulated by distinct mechanisms even though they share many functional characteristics.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Estrogen Receptor beta antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

The C-terminus fragment of recombinant human Estrogen Receptor beta protein was used as the immunogen for this antibody.

Storage

Store the Estrogen Receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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