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Home >> Antibodies >> Estrogen Receptor alpha Antibody

Estrogen Receptor alpha Antibody [clone NR3Ga-4] (V7560)

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  Catalog No Formulation Size Price (USD)  
Image V7560-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7560-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7560SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7560IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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IHC staining of FFPE human breast carcinoma with Estrogen Receptor alpha antibody (clone NR3Ga-4). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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IHC staining of FFPE human breast carcinoma with Estrogen Receptor alpha antibody (clone NR3Ga-4). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Immunofluorescence staining of human PFA-fixed MCF-7 cells with Estrogen Receptor alpha antibody (green, clone NR3Ga-4) and Phalloidin (red).
Flow cytometry testing of fixed and permeabilized human MCF-7 cells with Estrogen Receptor alpha antibody (clone NR3Ga-4); Red=isotype control, Blue= Estrogen Receptor alpha antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name NR3Ga-4
Purity Protein G affinity chromatography
UniProt P03372
Localization Nucleus
Applications Immunofluorescence : 1-2ug/ml
Flow cytometry : 1-2ug/10^6 cells
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This Estrogen Receptor alpha antibody is available for research use only.
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Description

Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. ER presence in breast tumors indicates an increased likelihood of response to anti-estrogen therapy.

Application Notes

Optimal dilution of the Estrogen Receptor alpha antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A recombinant human ESR1 protein fragment within amino acids 129-312 was used as the immunogen for the Estrogen Receptor alpha antibody.

Storage

Store the Estrogen Receptor alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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