Estrogen Receptor alpha Antibody FACS Intracellular Detection [clone ESR1/3559] (V7751)

Estrogen Receptor alpha Antibody for FACS MCF7 Cell. Flow cytometry analysis of permeabilized human MCF7 cells using Estrogen Receptor alpha antibody shows a clear rightward shift in fluorescence intensity compared to the isotype control, consistent with intracellular detection of ESR1 in hormone-responsive breast cancer cells; red histogram represents isotype control and blue histogram represents Estrogen Receptor alpha antibody staining, with detection performed using clone ESR1/3559.

Estrogen Receptor alpha Antibody Breast Carcinoma IHC. Immunohistochemistry of FFPE human breast carcinoma tissue using Estrogen Receptor alpha antibody shows strong HRP-DAB brown nuclear staining in tumor epithelial cells, consistent with ESR1 expression in hormone-responsive breast cancer, while surrounding stromal cells display minimal background; detection was performed with clone ESR1/3559. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Estrogen Receptor alpha antibody (clone ESR1/3559). These results demonstrate the foremost specificity of the ESR1/3559 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Estrogen Receptor alpha antibody (clone ESR1/3559) as confirmation of integrity and purity.

Estrogen Receptor alpha Antibody T-47D WB. Western blot analysis of human T-47D cell lysate using Estrogen Receptor alpha antibody detects a band at approximately 65-70 kDa, consistent with the predicted molecular weight of Estrogen receptor alpha (ESR1), a nuclear hormone receptor expressed in hormone-responsive breast cancer cells; detection was performed with clone ESR1/3559.

Estrogen Receptor alpha Antibody Breast Cancer Cell WB. Western blot analysis of human T-47D cell lysate using Estrogen Receptor alpha antibody detects a band at approximately 65-70 kDa, consistent with the predicted molecular weight of Estrogen receptor alpha (ESR1), a nuclear hormone receptor expressed in hormone-responsive breast cancer cells; detection was performed with clone ESR1/3559.

Estrogen Receptor alpha Antibody MCF7 WB. Western blot analysis of human MCF7 cell lysate using Estrogen Receptor alpha Antibody / Hormone Receptor and Breast Cancer Marker Antibody (clone ESR1/3559) detects a band at approximately 65-70 kDa, consistent with the predicted molecular weight of Estrogen receptor alpha (ESR1), a ligand-activated nuclear receptor expressed in breast cancer cells.