Estrogen Receptor alpha Antibody Microarray Validated |V7564

	Catalog No	Formulation	Size	Price (USD)
	V7564-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	559
Formulation
	V7564-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	259
Formulation
	V7564SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation
	V7564IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	559
Formulation

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Immunofluorescence analysis of Estrogen Receptor alpha antibody microarray validated clone ESR1/3557 in human MCF-7 cells. Formalin-fixed human MCF-7 breast carcinoma cells show strong nuclear green fluorescence using clone ESR1/3557, consistent with Estrogen Receptor alpha / ESR1 localization. Phalloidin (red) highlights actin filaments within the cytoskeleton. The nuclear staining pattern aligns with expected ER alpha distribution in hormone-responsive breast cancer cells.

IHC staining of FFPE human breast carcinoma with Estrogen Receptor alpha antibody (clone ESR1/3557). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Flow cytometry testing of fixed and permeabilized human MCF-7 cells with Estrogen Receptor alpha antibody (clone ESR1/3557); Red=isotype control, Blue= Estrogen Receptor alpha antibody.

Human protein microarray specificity validation of Estrogen Receptor alpha antibody microarray validated clone ESR1/3557. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins was performed using Estrogen Receptor alpha antibody clone ESR1/3557. The antibody demonstrates strongest binding to ESR1 with a Z-score of 139.43 and an S-score of 119.58, indicating clear separation from other proteins on the array. These results support the high specificity of clone ESR1/3557 for Estrogen Receptor alpha.
Z- and S-score explanation: The Z-score represents the strength of signal produced when the antibody, in combination with a fluorescently tagged anti-IgG secondary antibody, binds to a specific protein on the HuProt(TM) array. Z-scores are expressed in standard deviations above the mean signal generated across the array. Proteins are ranked in descending order based on Z-score. The S-score represents the difference in Z-scores between sequentially ranked proteins and reflects the relative specificity of the antibody for its intended target compared to potential off-target interactions.

SDS-PAGE analysis of purified, BSA-free Estrogen Receptor alpha antibody (clone ESR1/3557) as confirmation of integrity and purity.

Availability	1-3 business days
Species Reactivity	Human
Format	Purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	ESR1/3557
Purity	Protein G affinity chromatography
UniProt	P03372
Localization	Nucleus
Applications	Immunofluorescence : 1-2ug/ml Flow Cytometry : 1-2ug/10^6 cells Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations	This Estrogen Receptor alpha antibody is available for research use only.
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Description

Estrogen Receptor alpha Antibody Microarray Validated clone ESR1/3557 recognizes Estrogen Receptor alpha, a ligand-activated nuclear transcription factor encoded by the ESR1 gene on chromosome 6q25.1. Estrogen Receptor alpha, commonly referred to as ER alpha or ESR1, is a member of the nuclear receptor superfamily and functions as a transcriptional regulator in response to estrogen signaling. This mouse monoclonal antibody has undergone protein microarray specificity assessment to support selective recognition of ESR1 among a broad range of human proteins.

Estrogen Receptor alpha contains multiple functional domains, including a DNA-binding domain composed of zinc finger motifs, a hinge region involved in nuclear localization, and a ligand-binding domain that mediates estrogen interaction. Upon ligand binding, ESR1 undergoes conformational changes that promote receptor dimerization and recruitment of transcriptional coactivators or corepressors. These molecular interactions enable ER alpha to bind estrogen response elements within promoter regions of target genes and regulate transcriptional programs controlling proliferation, differentiation, and survival in hormone-responsive tissues.

Expression of ER alpha is most prominent in breast epithelium, endometrium, ovary, and other reproductive tissues. In oncology research, ESR1 expression is a central biomarker in breast carcinoma and additional estrogen-dependent malignancies. Alterations in ESR1 signaling, including gene amplification, splice variants, or activating mutations, can influence tumor growth behavior and endocrine responsiveness. Beyond oncology, ER alpha also contributes to bone metabolism, cardiovascular regulation, and neuroendocrine signaling pathways.

Microarray validation of clone ESR1/3557 provides an orthogonal assessment of target specificity by evaluating antibody binding across thousands of full-length human proteins. Such high-density protein array analysis supports confidence in selective ESR1 recognition in research settings. Estrogen Receptor alpha Antibody Microarray Validated clone ESR1/3557 enables investigation of ER alpha localization and expression in cellular and tissue-based models focused on estrogen-driven transcriptional regulation and hormone-dependent disease mechanisms.

Application Notes

Optimal dilution of the Estrogen Receptor alpha antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A recombinant human ESR1 protein fragment within amino acids 129-312 was used as the immunogen for the Estrogen Receptor alpha antibody microarray validated clone ESR1/3557.

Storage

Store the Estrogen Receptor alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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