Estrogen Receptor alpha Antibody [clone ESR1/3557] (V7564)

Immunofluorescence staining of human MCF-7 cells with Estrogen Receptor alpha antibody (green, clone ESR1/3557) and Phalloidin (red).

IHC staining of FFPE human breast carcinoma with Estrogen Receptor alpha antibody (clone ESR1/3557). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma with Estrogen Receptor alpha antibody (clone ESR1/3557). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Flow cytometry testing of fixed and permeabilized human MCF-7 cells with Estrogen Receptor alpha antibody (clone ESR1/3557); Red=isotype control, Blue= Estrogen Receptor alpha antibody.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Estrogen Receptor alpha antibody (clone ESR1/3557). These results demonstrate the foremost specificity of the ESR1/3557 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Estrogen Receptor alpha antibody (clone ESR1/3557) as confirmation of integrity and purity.