ERO1A Antibody / ERO1-like protein alpha Antibody (FY13585)

ERO1A Antibody by IHC in human esophageal carcinoma. Immunohistochemistry of paraffin-embedded human esophageal carcinoma using ERO1-like protein alpha Antibody shows prominent brown cytoplasmic staining in tumor epithelial cells, with variation in signal intensity across the section. Nuclei are counterstained blue with hematoxylin. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum. Primary antibody was applied at 2 ug/mL overnight at 4oC, followed by a peroxidase-conjugated secondary antibody and DAB chromogenic detection.

ERO1A Antibody by WB in human and rodent cell lines. Western blot analysis using ERO1-like protein alpha Antibody detects a band near 54 kDa in human A431 (lane 1), HeLa (lane 2), HepG2 (lane 3), 293T (lane 4), rat SHZ-88 (lane 5), and mouse RAW264.7 (lane 6). The calculated molecular weight is approximately 54.4 kDa. Reduced samples containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. Membranes were blocked with 5% nonfat milk and incubated with primary antibody at 0.5 ug/mL overnight at 4oC. An HRP-conjugated secondary antibody and chemiluminescent substrate were used for detection.