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Home >> Antibodies >> ERK2 Antibody

ERK2 Antibody (R30368)

  Catalog No Formulation Size Price (USD)  
Image R30368 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of ERK2 antibody and Lane 1: human placenta; 2: rat liver; 3: rat brain; 4: rat thymus; 5: HeLa; 6: SMMC-7721; 7: COLO320; 8: MM231; 9: CEM
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P28482
Applications Western blot : 0.5-1ug/ml
Limitations This ERK2 antibody is available for research use only.
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Description

Mitogen-activated protein kinase 1, also known as MAPK1, p42MAPK, and ERK2, is an enzyme which in humans is encoded by the MAPK1 gene. The gene is mapped to 22q11.2. The protein encoded by this gene is a member of the MAP kinase family. ERK2 is widely involved in eukaryotic signal transduction. Upon activation, it translocates to the nucleus of the stimulated cell, where it phosphorylates nuclear targets. Futhermore, the ERK pathway is necessary for experience-dependent plasticity and for long-term potentiation of synaptic transmission in the developing visual cortex. The pathway is also involved in responses to NTN1.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the ERK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of human ERK2 (IAEAPFKFDMEL) was used as the immunogen for this ERK2 antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the ERK2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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