ERGIC2 Antibody / ERGIC and golgi 2 Antibody (FY13630)

ERGIC2 Antibody by IF in U2OS cells. Immunofluorescence analysis using ERGIC and golgi 2 Antibody reveals strong green nuclear fluorescence with a weaker cytoplasmic signal in U2OS cells. Red alpha-tubulin counterstaining highlights the cytoskeleton. Cells were treated with enzyme-based antigen retrieval and incubated with 5 ug/mL primary antibody overnight at 4oC before fluorescent secondary antibody detection. The image depicts the observed staining pattern without establishing organelle-specific colocalization.

ERGIC2 Antibody by IHC in human prostate carcinoma. Immunohistochemistry of paraffin-embedded human prostate carcinoma using ERGIC and golgi 2 Antibody shows brown staining in glandular epithelial structures with hematoxylin-counterstained nuclei. Heat-mediated antigen retrieval was performed using EDTA buffer at pH 8.0. Sections were blocked with goat serum and incubated with 2 ug/mL primary antibody overnight at 4oC, followed by HRP-conjugated secondary antibody and DAB visualization.

ERGIC2 Antibody by IHC in human lung carcinoma. Immunohistochemistry of paraffin-embedded human lung carcinoma using ERGIC and golgi 2 Antibody shows brown staining in tumor cells and adjacent tissue structures with hematoxylin-counterstained nuclei. Heat-mediated antigen retrieval was performed using EDTA buffer at pH 8.0. Sections were blocked with goat serum and incubated with 2 ug/mL primary antibody overnight at 4oC, followed by HRP-conjugated secondary antibody and DAB visualization.

ERGIC2 Antibody by WB in human PC-3 and HeLa cells. Western blot analysis using ERGIC and golgi 2 Antibody detects a prominent immunoreactive band consistent with the expected molecular weight of approximately 43 kDa. Lanes 1 and 2 contain PC-3 and HeLa whole-cell lysates, respectively. Reduced samples containing 30 ug total protein per lane were resolved by 10% SDS-PAGE and transferred to nitrocellulose. The membrane was blocked with 5% nonfat milk, incubated with 0.5 ug/mL primary antibody overnight at 4oC, and probed with an HRP-conjugated secondary antibody. Signal was visualized by chemiluminescence.

ERGIC2 Antibody by IP in HeLa. Immunoprecipitation of ERGIC and golgi 2 Antibody from HeLa whole-cell lysate was assessed by Western blot. Lane 1 contains 30 ug input lysate, lane 2 a rabbit IgG control immunoprecipitate, and lane 3 the antibody-specific immunoprecipitate prepared with 2 ug antibody and 500 ug lysate. An immunoreactive band consistent with the expected approximately 43 kDa protein is visible in input and the specific immunoprecipitate. The 50 kDa signal in immunoprecipitation lanes is consistent with immunoglobulin heavy chain.