eRF1 Antibody | Translation Release Factor

	Catalog No	Formulation	Size	Price (USD)
	RQ6231	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

eRF1 Antibody Human Breast Cancer IHC. Immunohistochemical staining of FFPE human breast cancer tissue using eRF1 Antibody / Translation Release Factor Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8.0 EDTA buffer for 20 minutes followed by cooling before staining. eRF1 exhibits predominantly cytoplasmic staining in tumor cells, consistent with its role as the universal translation release factor that mediates translation termination at the ribosome. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for investigating translation termination, protein synthesis, and translational regulation.

eRF1 Antibody Human Tonsil IHC. Immunohistochemical staining of FFPE human tonsil tissue using eRF1 Antibody / Translation Release Factor Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8.0 EDTA buffer for 20 minutes followed by cooling before staining. eRF1 exhibits predominantly cytoplasmic staining in lymphoid cells, consistent with its ubiquitous expression and essential role as the universal translation release factor during protein synthesis. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for investigating translation termination, ribosome function, and translational regulation.

eRF1 Antibody Human Ovarian Cancer IHC. Immunohistochemical staining of FFPE human ovarian cancer tissue using eRF1 Antibody / Translation Release Factor Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8.0 EDTA buffer for 20 minutes followed by cooling before staining. eRF1 shows predominantly cytoplasmic staining in tumor cells, consistent with its essential role as the universal translation release factor that mediates translation termination during protein synthesis. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for investigating translation termination, ribosome function, and translational regulation.

eRF1 Antibody Human Lung Cancer IHC. Immunohistochemical staining of FFPE human lung cancer tissue using eRF1 Antibody / Translation Release Factor Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8.0 EDTA buffer for 20 minutes followed by cooling before staining. eRF1 exhibits predominantly cytoplasmic staining in tumor cells, consistent with its essential role as the universal translation release factor that mediates translation termination during protein synthesis. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for investigating translation termination, ribosome function, and translational regulation.

eRF1 Antibody Human Liver Cancer IHC. Immunohistochemical staining of FFPE human liver cancer tissue using eRF1 Antibody / Translation Release Factor Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8.0 EDTA buffer for 20 minutes followed by cooling before staining. eRF1 exhibits predominantly cytoplasmic staining in tumor cells, consistent with its essential role as the universal translation release factor that mediates translation termination during protein synthesis. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for investigating translation termination, ribosome function, and translational regulation.

eRF1 Antibody Rat Kidney IHC. Immunohistochemical staining of FFPE rat kidney tissue using eRF1 Antibody / Translation Release Factor Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8.0 EDTA buffer for 20 minutes followed by cooling before staining. eRF1 exhibits predominantly cytoplasmic staining in renal tubular epithelial cells, consistent with its ubiquitous expression and essential role as the universal translation release factor that mediates translation termination during protein synthesis. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for investigating translation termination, ribosome function, and translational regulation.

eRF1 Antibody Human MCF7 Cell IF. Immunofluorescent staining of FFPE human MCF7 cells using eRF1 Antibody / Translation Release Factor Antibody (green) with DAPI nuclear counterstain (blue). Heat-induced epitope retrieval was performed by steaming tissue sections in pH 6.0 citrate buffer for 20 minutes before staining. eRF1 displays predominantly cytoplasmic staining, consistent with its role as the universal translation release factor that functions at the ribosome during protein synthesis. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for studies of translation termination, ribosome function, and translational regulation.

eRF1 Antibody Human, Rat and Mouse WB. Western blot testing of human HeLa (lane 1), Jurkat (lane 2), K562 (lane 3), Raji (lane 4), and HepG2 (lane 5) cell lysates, rat PC-12 (lane 6), and mouse RAW264.7 cell lysate (lane 7) using eRF1 Antibody / Translation Release Factor Antibody at 1 ug/ml. A specific band is detected at approximately 49 kDa in all samples, consistent with the predicted molecular weight of eRF1 (ETF1). This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for studies of translation termination, ribosome function, protein synthesis, and translational regulation.

eRF1 Antibody Human Caco-2 Cell FACS. Flow cytometry analysis of fixed and permeabilized human Caco-2 cells stained with eRF1 Antibody / Translation Release Factor Antibody (blue). Cells were blocked with goat serum and incubated with eRF1 Antibody at 1 ug/million cells, followed by a fluorescent secondary antibody. Unstained cells (red) and an isotype control (green) were included to assess background staining. The clear rightward shift of the eRF1-positive population demonstrates specific intracellular detection. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for flow cytometric analysis of intracellular eRF1 expression.

eRF1 Antibody Mouse HEPA1-6 Cell FACS. Flow cytometry analysis of fixed and permeabilized mouse HEPA1-6 cells stained with eRF1 Antibody / Translation Release Factor Antibody (blue). Cells were blocked with goat serum and incubated with eRF1 Antibody at 1 ug/million cells, followed by a fluorescent secondary antibody. Unstained cells (red) and an isotype control (green) were included to assess background staining. The pronounced rightward shift of the eRF1-positive population demonstrates specific intracellular detection. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for flow cytometric analysis of intracellular eRF1 expression.

eRF1 Antibody Rat RH35 Cell FACS. Flow cytometry analysis of fixed and permeabilized rat RH35 cells stained with eRF1 Antibody / Translation Release Factor Antibody (blue). Cells were blocked with goat serum and incubated with eRF1 Antibody at 1 ug/million cells, followed by a fluorescent secondary antibody. Unstained cells (red) and an isotype control (green) were included to assess background staining. The pronounced rightward shift of the eRF1-positive population demonstrates specific intracellular detection. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for flow cytometric analysis of intracellular eRF1 expression.

›

eRF1 Antibody Human Breast Cancer IHC. Immunohistochemical staining of FFPE human breast cancer tissue using eRF1 Antibody / Translation Release Factor Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8.0 EDTA buffer for 20 minutes followed by cooling before staining. eRF1 exhibits predominantly cytoplasmic staining in tumor cells, consistent with its role as the universal translation release factor that mediates translation termination at the ribosome. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for investigating translation termination, protein synthesis, and translational regulation.

eRF1 Antibody Human Breast Cancer IHC. Immunohistochemical staining of FFPE human breast cancer tissue using eRF1 Antibody / Translation Release Factor Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8.0 EDTA buffer for 20 minutes followed by cooling before staining. eRF1 shows predominantly cytoplasmic staining in tumor cells, consistent with its essential role as the universal translation release factor that mediates translation termination during protein synthesis. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for investigating translation termination, ribosome function, and translational regulation.

eRF1 Antibody Human Tonsil IHC. Immunohistochemical staining of FFPE human tonsil tissue using eRF1 Antibody / Translation Release Factor Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8.0 EDTA buffer for 20 minutes followed by cooling before staining. eRF1 exhibits predominantly cytoplasmic staining in lymphoid cells, consistent with its ubiquitous expression and essential role as the universal translation release factor during protein synthesis. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for investigating translation termination, ribosome function, and translational regulation.

eRF1 Antibody Human Ovarian Cancer IHC. Immunohistochemical staining of FFPE human ovarian cancer tissue using eRF1 Antibody / Translation Release Factor Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8.0 EDTA buffer for 20 minutes followed by cooling before staining. eRF1 shows predominantly cytoplasmic staining in tumor cells, consistent with its essential role as the universal translation release factor that mediates translation termination during protein synthesis. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for investigating translation termination, ribosome function, and translational regulation.

eRF1 Antibody Human Lung Cancer IHC. Immunohistochemical staining of FFPE human lung cancer tissue using eRF1 Antibody / Translation Release Factor Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8.0 EDTA buffer for 20 minutes followed by cooling before staining. eRF1 exhibits predominantly cytoplasmic staining in tumor cells, consistent with its essential role as the universal translation release factor that mediates translation termination during protein synthesis. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for investigating translation termination, ribosome function, and translational regulation.

eRF1 Antibody Human Liver Cancer IHC. Immunohistochemical staining of FFPE human liver cancer tissue using eRF1 Antibody / Translation Release Factor Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8.0 EDTA buffer for 20 minutes followed by cooling before staining. eRF1 exhibits predominantly cytoplasmic staining in tumor cells, consistent with its essential role as the universal translation release factor that mediates translation termination during protein synthesis. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for investigating translation termination, ribosome function, and translational regulation.

eRF1 Antibody Rat Kidney IHC. Immunohistochemical staining of FFPE rat kidney tissue using eRF1 Antibody / Translation Release Factor Antibody. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8.0 EDTA buffer for 20 minutes followed by cooling before staining. eRF1 exhibits predominantly cytoplasmic staining in renal tubular epithelial cells, consistent with its ubiquitous expression and essential role as the universal translation release factor that mediates translation termination during protein synthesis. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for investigating translation termination, ribosome function, and translational regulation.

eRF1 Antibody Human MCF7 Cell IF. Immunofluorescent staining of FFPE human MCF7 cells using eRF1 Antibody / Translation Release Factor Antibody (green) with DAPI nuclear counterstain (blue). Heat-induced epitope retrieval was performed by steaming tissue sections in pH 6.0 citrate buffer for 20 minutes before staining. eRF1 displays predominantly cytoplasmic staining, consistent with its role as the universal translation release factor that functions at the ribosome during protein synthesis. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for studies of translation termination, ribosome function, and translational regulation.

eRF1 Antibody Human, Rat and Mouse WB. Western blot testing of human HeLa (lane 1), Jurkat (lane 2), K562 (lane 3), Raji (lane 4), and HepG2 (lane 5) cell lysates, rat PC-12 (lane 6), and mouse RAW264.7 cell lysate (lane 7) using eRF1 Antibody / Translation Release Factor Antibody at 1 ug/ml. A specific band is detected at approximately 49 kDa in all samples, consistent with the predicted molecular weight of eRF1 (ETF1). This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for studies of translation termination, ribosome function, protein synthesis, and translational regulation.

eRF1 Antibody Human Caco-2 Cell FACS. Flow cytometry analysis of fixed and permeabilized human Caco-2 cells stained with eRF1 Antibody / Translation Release Factor Antibody (blue). Cells were blocked with goat serum and incubated with eRF1 Antibody at 1 ug/million cells, followed by a fluorescent secondary antibody. Unstained cells (red) and an isotype control (green) were included to assess background staining. The clear rightward shift of the eRF1-positive population demonstrates specific intracellular detection. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for flow cytometric analysis of intracellular eRF1 expression.

eRF1 Antibody Mouse HEPA1-6 Cell FACS. Flow cytometry analysis of fixed and permeabilized mouse HEPA1-6 cells stained with eRF1 Antibody / Translation Release Factor Antibody (blue). Cells were blocked with goat serum and incubated with eRF1 Antibody at 1 ug/million cells, followed by a fluorescent secondary antibody. Unstained cells (red) and an isotype control (green) were included to assess background staining. The pronounced rightward shift of the eRF1-positive population demonstrates specific intracellular detection. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for flow cytometric analysis of intracellular eRF1 expression.

eRF1 Antibody Rat RH35 Cell FACS. Flow cytometry analysis of fixed and permeabilized rat RH35 cells stained with eRF1 Antibody / Translation Release Factor Antibody (blue). Cells were blocked with goat serum and incubated with eRF1 Antibody at 1 ug/million cells, followed by a fluorescent secondary antibody. Unstained cells (red) and an isotype control (green) were included to assess background staining. The pronounced rightward shift of the eRF1-positive population demonstrates specific intracellular detection. This eRF1 Antibody, also called Translation Release Factor Antibody, is suitable for flow cytometric analysis of intracellular eRF1 expression.

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG2a
Clone Name	3B6
Purity	Affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	P62495
Localization	Cytoplasmic
Applications	Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml Flow Cytometry : 1-3ug/million cells
Limitations	This eRF1 Antibody / Translation Release Factor Antibody is available for research use only.
	Review this product on BioCompare and get a $20 Amazon gift card

Description

eRF1 Antibody / Translation Release Factor Antibody, clone 3B6, is a mouse monoclonal antibody that recognizes eRF1, the protein encoded by the ETF1 gene and the essential translation release factor responsible for recognizing all three stop codons during eukaryotic protein synthesis. Acting together with the GTPase eRF3, eRF1 promotes hydrolysis of the peptidyl-tRNA bond within the ribosome, allowing release of newly synthesized polypeptides and completion of translation. Because it recognizes the UAA, UAG, and UGA stop codons, eRF1 serves as the universal class I release factor required for efficient and accurate translation termination.

eRF1 contains conserved functional domains that mediate stop codon recognition, ribosome binding, and peptide release. In addition to terminating translation, eRF1 contributes to ribosome recycling and mRNA quality control pathways, including nonsense-mediated decay, helping maintain translational fidelity and prevent production of aberrant proteins. Through these activities, eRF1 plays a central role in protein homeostasis and regulation of gene expression at the translational level.

Because translation termination occurs in every actively translating cell, eRF1 is ubiquitously expressed across tissues and cell types. Alterations in eRF1 function can influence stop codon readthrough, translational accuracy, cellular stress responses, and disease-associated protein synthesis. Consequently, eRF1 is widely studied in molecular biology, cancer research, virology, and investigations of genetic disorders caused by premature termination codons. These studies have also established eRF1 as an important target for therapeutic strategies designed to modulate translation termination and restore functional protein expression.

An eRF1 Antibody is a valuable reagent for investigating translation termination, ribosome-associated protein complexes, and translational fidelity. Researchers frequently use Translation Release Factor Antibody reagents to study protein synthesis, stop codon recognition, ribosome biology, mRNA surveillance, and mechanisms that regulate translational control. Characterization of clone 3B6 supports its use for investigating eRF1 expression in research applications validated for this antibody.

Learn more about the official gene nomenclature, biological functions, and additional validation data by visiting our ETF1 Antibody / Eukaryotic Translation Termination Factor Antibody page.

Application Notes

Optimal dilution of the eRF1 Antibody / Translation Release Factor Antibody should be determined by the researcher.

Immunogen

A human recombinant partial protein (amino acids D9-K342) was used as the immunogen for the eRF1 antibody.

Storage

After reconstitution, the eRF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

eRF1 Antibody, Translation Release Factor Antibody, ETF1 Antibody, Eukaryotic Translation Termination Factor 1 Antibody, Eukaryotic Peptide Chain Release Factor 1 Antibody

Related Products

1. View all ETF1 antibodies

eRF1 Antibody / Translation Release Factor Antibody [clone 3B6] (RQ6231)

Description

Application Notes

Immunogen

Storage

Alternate Names

Related Products

Area of Research

Product Type

Protocols

Contact Us

NSJ Bioreagents