- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
ERCC1 Antibody / Tissue Expression Antibody [clone ERCC1/7597] (V4635)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V4635-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
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V4635-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
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V4635SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | ERCC1/7597 |
| Purity | Protein A/G affinity |
| UniProt | P07992 |
| Localization | Nucleus |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT Western Blot : 2-4ug/ml |
| Limitations | This ERCC1 Antibody / Tissue Expression Antibody is available for research use only. |
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Excision repair cross-complementation group 1 (ERCC1) is a nuclear DNA repair protein that plays an essential role in the nucleotide excision repair pathway, where it facilitates removal of DNA lesions caused by environmental and endogenous damage. The ERCC1 antibody detects expression of this protein across multiple tissue types, enabling evaluation of DNA repair activity in both normal and diseased states.
ERCC1 functions as part of a heterodimeric complex with XPF, forming a structure-specific endonuclease that cleaves DNA at sites of damage. This activity is critical for excision of damaged nucleotides and subsequent repair synthesis. In addition to nucleotide excision repair, ERCC1 contributes to interstrand crosslink repair and maintenance of genomic stability, supporting cellular survival under conditions of DNA stress.
Expression of ERCC1 is widely distributed across human tissues, with nuclear localization reflecting its role in DNA repair processes. Tissue-specific differences in expression levels may be observed, depending on cellular proliferation rate, exposure to DNA-damaging agents, and metabolic activity. Immunohistochemical analysis typically reveals nuclear staining in epithelial cells, consistent with active DNA repair mechanisms.
In cancer research, ERCC1 expression is frequently evaluated in tumor tissues due to its association with DNA repair capacity and therapeutic response. Variations in ERCC1 levels have been linked to differences in sensitivity to DNA-damaging chemotherapy agents, making it an important marker for studies of tumor biology and treatment resistance.
Western blot analysis further supports detection of ERCC1 across tissue lysates, where a band corresponding to the expected molecular weight is observed. This allows comparison of expression levels between tissues and experimental conditions, complementing findings from immunohistochemistry.
The ERCC1/7597 clone supports analysis of tissue distribution and relative expression patterns, making it suitable for studies focused on DNA repair activity across different biological contexts. Detection of ERCC1 expression contributes to understanding of genomic stability, cellular stress responses, and tumor heterogeneity.
For broader detection of ERCC1 as a DNA repair marker, see our ERCC1 antibody.
Optimal dilution of the ERCC1 Antibody / Tissue Expression Antibody should be determined by the researcher.
A recombinant partial protein sequence (within amino acids 191-281) from the human protein was used as the immunogen for the ERCC1 antibody.
Aliquot the ERCC1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
ERCC1 antibody, ERCC1 tissue expression antibody, DNA repair protein ERCC1 antibody, ERCC1 nuclear staining antibody
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