- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
ErbB2 Antibody / HER2 / CF488 Conjugate [clone HRB2/258] (V2109CF488)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V2109CF488-100T | 500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 Tests | 539 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | CF488 Conjugate |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2a, kappa |
| Clone Name | HRB2/258 |
| Purity | Protein G affinity chromatography |
| Localization | Cell surface |
| Applications | Flow Cytometry : 1-2ug/10^6 cells Immunofluorescence : 1-3ug/ml |
| Limitations | This ErbB2 antibody is available for research use only. |
|
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ErbB2 antibody CF488 Conjugate detects Receptor tyrosine-protein kinase erbB-2, also known as HER2, a membrane-bound receptor tyrosine kinase that regulates cellular growth, survival, and differentiation. The UniProt recommended name is Receptor tyrosine-protein kinase erbB-2 (ERBB2). This CF488-conjugated antibody allows direct fluorescence detection of HER2/ErbB2 expression in cells and tissues without the need for a secondary antibody, providing precise visualization of receptor localization and abundance.
Functionally, ErbB2 antibody CF488 Conjugate identifies a 185 kDa single-pass transmembrane protein that forms heterodimers with other members of the ERBB family, including EGFR (ERBB1), ERBB3, and ERBB4. These receptor interactions activate key intracellular signaling cascades such as the MAPK/ERK and PI3K/AKT pathways, driving cellular proliferation and inhibiting apoptosis. HER2/ErbB2 lacks a known ligand but serves as the preferred dimerization partner for other ERBB receptors, amplifying growth and survival signaling under both normal and pathological conditions.
The ERBB2 gene is located on chromosome 17q12 and is expressed in epithelial tissues of the heart, mammary gland, and gastrointestinal tract. While normally expressed at low levels, gene amplification or protein overexpression causes constitutive activation of mitogenic signaling. Elevated HER2/ErbB2 expression is a defining feature of several cancers, including breast, ovarian, and gastric carcinomas, where it contributes to tumor aggressiveness and poor prognosis.
The CF488-conjugated ErbB2 antibody provides bright, photostable green fluorescence ideal for use in immunofluorescence microscopy and flow cytometry. This format simplifies multi-label assays and enables co-localization studies with other cellular markers. Research applications include quantifying HER2 receptor density, studying receptor trafficking, and visualizing HER2 expression in tumor tissues.
ErbB2 antibody CF488 Conjugate is validated for use in relevant fluorescence-based research applications to detect HER2/ErbB2 in cell and tissue models. NSJ Bioreagents provides this conjugated antibody optimized for studies in cancer signaling, receptor localization, and HER2-targeted therapeutic research.
Optimal dilution of the ErbB2 antibody should be determined by the researcher.
Recombinant human protein was used as the immunogen for this ErbB2 antibody. Its epitope is localized in the extracellular domain.
Store the ErbB2 antibody at 2-8oC, protected from light.
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